Overview

  • Product name

  • Description

    Rabbit polyclonal to ABHD2
  • Host species

    Rabbit
  • Specificity

    ab74680 detects endogenous levels of total ABHD2 protein.
  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal sequence of human ABHD2.

  • Positive control

    • HT29 cell extracts and MCF7 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab74680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
ELISA 1/40000.
ICC/IF 1/500 - 1/1000.

Target

  • Relevance

    ABHD2 contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. The function ABHD2 remains unknown but it may play a role in smooth muscle cell migration. Alternative splicing of this gene results in two transcript variants encoding the same protein.
  • Cellular localization

    Membrane; Single-pass type II membrane protein
  • Database links

  • Alternative names

    • 2210009N18Rik antibody
    • abhydrolase domain containing 2 antibody
    • Abhydrolase domain-containing protein 2 antibody
    • alpha/beta hydrolase domain containing protein 2 antibody
    • HS1 2 antibody
    • Labh 2 antibody
    • LABH2 antibody
    • Lung alpha/beta hydrolase 2 antibody
    • MGC107122 antibody
    • MGC111112 antibody
    • MGC26249 antibody
    • PHPS1 2 antibody
    • protein PHPS1-2 antibody
    see all

Images

  • ab74680 at 1/500-1/1000 dilution staining ABHD2 in MCF7 cells by Immunofluorescence, in the absence (-) or presence (+) of the immunising peptide.
  • All lanes : Anti-ABHD2 antibody (ab74680) at 1/500 dilution

    Lane 1 : HT29 cell extracts
    Lane 2 : HT29 cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 49 kDa
    Observed band size: 49 kDa
    Additional bands at: 100 kDa, 26 kDa, 95 kDa. We are unsure as to the identity of these extra bands.

References

ab74680 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab74680.
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