Product nameAnti-ABHD2 antibody
See all ABHD2 primary antibodies
DescriptionRabbit polyclonal to ABHD2
Specificityab74680 detects endogenous levels of total ABHD2 protein.
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide derived from an internal sequence of human ABHD2.
- HT29 cell extracts and MCF7 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).|
|ICC/IF||1/500 - 1/1000.|
RelevanceABHD2 contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. The function ABHD2 remains unknown but it may play a role in smooth muscle cell migration. Alternative splicing of this gene results in two transcript variants encoding the same protein.
Cellular localizationMembrane; Single-pass type II membrane protein
- 2210009N18Rik antibody
- abhydrolase domain containing 2 antibody
- Abhydrolase domain-containing protein 2 antibody
ab74680 at 1/500-1/1000 dilution staining ABHD2 in MCF7 cells by Immunofluorescence, in the absence (-) or presence (+) of the immunising peptide.
All lanes : Anti-ABHD2 antibody (ab74680) at 1/500 dilution
Lane 1 : HT29 cell extracts
Lane 2 : HT29 cell extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Additional bands at: 100 kDa, 26 kDa, 95 kDa. We are unsure as to the identity of these extra bands.
ab74680 has not yet been referenced specifically in any publications.