Key features and details
- Rabbit polyclonal to ABHD2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ABHD2 antibody
See all ABHD2 primary antibodies
DescriptionRabbit polyclonal to ABHD2
Specificityab74680 detects endogenous levels of total ABHD2 protein.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide derived from an internal sequence of human ABHD2.
- HT29 cell extracts and MCF7 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).|
|ICC/IF||1/500 - 1/1000.|
RelevanceABHD2 contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. The function ABHD2 remains unknown but it may play a role in smooth muscle cell migration. Alternative splicing of this gene results in two transcript variants encoding the same protein.
Cellular localizationMembrane; Single-pass type II membrane protein
- 2210009N18Rik antibody
- abhydrolase domain containing 2 antibody
- Abhydrolase domain-containing protein 2 antibody
ab74680 at 1/500-1/1000 dilution staining ABHD2 in MCF7 cells by Immunofluorescence, in the absence (-) or presence (+) of the immunising peptide.
All lanes : Anti-ABHD2 antibody (ab74680) at 1/500 dilution
Lane 1 : HT29 cell extracts
Lane 2 : HT29 cell extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Additional bands at: 100 kDa, 26 kDa, 95 kDa. We are unsure as to the identity of these extra bands.
ab74680 has not yet been referenced specifically in any publications.