• Product name

    Anti-Abhd5/CGI-58 antibody
    See all Abhd5/CGI-58 primary antibodies
  • Description

    Goat polyclonal to Abhd5/CGI-58
  • Host species

  • Specificity

    This antibody is not expected to cross-react with ABHD4.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Cow, Human
    Predicted to work with: Rat, Sheep, Dog, Pig, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Abhd5/CGI-58 aa 181-192 (internal sequence).


  • Positive control

    • A431 and NIH-3T3 cell line lysates.
  • General notes

    Previously labelled as Abhd5. 



Our Abpromise guarantee covers the use of ab59488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa). Additional faint bands of 26kDa and 18kDa were observed in NIH-3T3. These bands were successfully blocked by incubation with the immunizing peptide.
ELISA Use at an assay dependent dilution. Peptide ELISA: antibody detection limit dilution 1:8,000.


  • Function

    Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation.
  • Tissue specificity

    Widely expressed in various tissues, including lymphocytes, liver, skeletal muscle and brain. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and neurons (at protein level).
  • Involvement in disease

    Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS) [MIM:275630]; also called triglyceride storage disease with impaired long-chain fatty acid oxidation or neutral lipid storage disease with ichthyosis. CDS is an autosomal recessive inborn error of lipid metabolism with multisystemic accumulation of triglycerides although plasma concentrations are normal. Clinical characteristics are congenital generalized ichthyosis, vacuolated leukocytes, hepatomegaly, myopathy, cataracts, neurosensory hearing loss and developmental delay. The disorder presents at birth with generalized, fine, white scaling of the skin and a variable degree of erythema resembling non-bullous congenital ichthyosiform erythroderma.
  • Sequence similarities

    Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.
  • Developmental stage

    Detected in fetal epidermis from 49 to 135 days estimated gestational age (at protein level).
  • Domain

    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
  • Cellular localization

    Cytoplasm. Lipid droplet. Colocalized with PLIN and ADRP on the surface of lipid droplets. The localization is dependent upon the metabolic status of the adipocytes and the activity of PKA.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1-acylglycerol-3-phosphate O-acyltransferase ABHD5 antibody
    • ABHD5 antibody
    • ABHD5_HUMAN antibody
    • Abhydrolase domain containing 5 antibody
    • Abhydrolase domain containing protein 5 antibody
    • Abhydrolase domain-containing protein 5 antibody
    • CDS antibody
    • CGI 58 antibody
    • CGI58 antibody
    • CGI58 protein antibody
    • IECN2 antibody
    • Lipid droplet-binding protein CGI-58 antibody
    • MGC8731 antibody
    • NCIE2 antibody
    see all


  • Anti-Abhd5/CGI-58 antibody (ab59488) at 1 µg/ml + A431 cell line lysate (35µg protein in RIPA buffer)

    Predicted band size: 39 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?

    Primary incubation was 1 hour. Detected by chemiluminescence.
  • Anti-Abhd5/CGI-58 antibody (ab59488) at 0.2 µg/ml + NIH-3T3 cell lysate (RIPA buffer) at 35 µg

    Predicted band size: 39 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?

    Primary incubation was 1 hour. Detected by chemiluminescence.


This product has been referenced in:

  • Lin YY  et al. KSRP and MicroRNA 145 Are Negative Regulators of Lipolysis in White Adipose Tissue. Mol Cell Biol 34:2339-2349 (2014). Read more (PubMed: 24732799) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab59488.
Please use the links above to contact us or submit feedback about this product.

For licensing inquiries, please contact partnerships@abcam.com

Sign up