Overview

  • Product name

    Anti-Abhd5/CGI-58 antibody
    See all Abhd5/CGI-58 primary antibodies
  • Description

    Rabbit polyclonal to Abhd5/CGI-58
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Pig, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Abhd5/CGI-58 aa 150-250 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab89382)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: HeLa; A431; MOLT4; SAOS 2.
  • General notes

    Previously labelled as Abhd5. 

Properties

Applications

Our Abpromise guarantee covers the use of ab73551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 25054327
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 39 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation.
  • Tissue specificity

    Widely expressed in various tissues, including lymphocytes, liver, skeletal muscle and brain. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and neurons (at protein level).
  • Involvement in disease

    Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS) [MIM:275630]; also called triglyceride storage disease with impaired long-chain fatty acid oxidation or neutral lipid storage disease with ichthyosis. CDS is an autosomal recessive inborn error of lipid metabolism with multisystemic accumulation of triglycerides although plasma concentrations are normal. Clinical characteristics are congenital generalized ichthyosis, vacuolated leukocytes, hepatomegaly, myopathy, cataracts, neurosensory hearing loss and developmental delay. The disorder presents at birth with generalized, fine, white scaling of the skin and a variable degree of erythema resembling non-bullous congenital ichthyosiform erythroderma.
  • Sequence similarities

    Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.
  • Developmental stage

    Detected in fetal epidermis from 49 to 135 days estimated gestational age (at protein level).
  • Domain

    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
  • Cellular localization

    Cytoplasm. Lipid droplet. Colocalized with PLIN and ADRP on the surface of lipid droplets. The localization is dependent upon the metabolic status of the adipocytes and the activity of PKA.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1-acylglycerol-3-phosphate O-acyltransferase ABHD5 antibody
    • ABHD5 antibody
    • ABHD5_HUMAN antibody
    • Abhydrolase domain containing 5 antibody
    • Abhydrolase domain containing protein 5 antibody
    • Abhydrolase domain-containing protein 5 antibody
    • CDS antibody
    • CGI 58 antibody
    • CGI58 antibody
    • CGI58 protein antibody
    • IECN2 antibody
    • Lipid droplet-binding protein CGI-58 antibody
    • MGC8731 antibody
    • NCIE2 antibody
    see all

Images

  • All lanes : Anti-Abhd5/CGI-58 antibody (ab73551) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
    Lane 4 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 39 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?


    Exposure time: 4 minutes
  • ICC/IF image of ab73551 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73551, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Lassen S  et al. Perilipin-2 is critical for efficient lipoprotein and hepatitis C virus particle production. J Cell Sci 132:N/A (2019). Read more (PubMed: 30559250) »
  • Mason RR  et al. Cellular localization and associations of the major lipolytic proteins in human skeletal muscle at rest and during exercise. PLoS One 9:e103062 (2014). IHC-Fr ; Human . Read more (PubMed: 25054327) »
See all 2 Publications for this product

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