Anti-ABHD6 antibody (ab74681)
Key features and details
- Rabbit polyclonal to ABHD6
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ABHD6 antibody -
Description
Rabbit polyclonal to ABHD6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human ABHD6 (internal sequence).
Database link: Q9BV23 -
Positive control
- COLO cell extracts and A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74681 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
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ICC/IF |
1/500 - 1/1000.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). |
ICC/IF
1/500 - 1/1000. |
Target
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Relevance
ABHD6 belongs to the alpha/beta hydrolase superfamily. It is highly expressed in mammalian brain and catalyzes the hydrolysis of the endocannabinoid 2-arachidonoyl glycerol (2-AG). -
Cellular localization
Membrane; Single-pass type II membrane protein -
Database links
- Entrez Gene: 57406 Human
- Entrez Gene: 66082 Mouse
- Entrez Gene: 305795 Rat
- SwissProt: Q9BV23 Human
- SwissProt: Q8R2Y0 Mouse
- SwissProt: Q5XI64 Rat
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Alternative names
- 0610041D24Rik antibody
- 2 arachidonoylglycerol hydrolase antibody
- AA673485 antibody
see all
Images
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ab74681 at 1/500-1/1000 dilution staining ABHD6 in A549 cells by Immunofluorescence, in the absence (-) or presence (+) of the immunising peptide.
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All lanes : Anti-ABHD6 antibody (ab74681) at 1/500 dilution
Lane 1 : COLO cell extracts
Lane 2 : COLO cell extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab74681 has been referenced in 1 publication.
- Tang Z et al. Enhanced monoacylglycerol lipolysis by ABHD6 promotes NSCLC pathogenesis. EBioMedicine 53:102696 (2020). PubMed: 32143183