Key features and details
- Rabbit polyclonal to ABHD6
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ABHD6 antibody
See all ABHD6 primary antibodies
DescriptionRabbit polyclonal to ABHD6
Specificityab74681 detects endogenous levels of total ABHD6 protein.
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide derived from an internal sequence of human ABHD6.
- COLO cell extracts and A549 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74681 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).|
|ICC/IF||1/500 - 1/1000.|
RelevanceABHD6 belongs to the alpha/beta hydrolase superfamily. It is highly expressed in mammalian brain and catalyzes the hydrolysis of the endocannabinoid 2-arachidonoyl glycerol (2-AG).
Cellular localizationMembrane; Single-pass type II membrane protein
- 0610041D24Rik antibody
- 2 arachidonoylglycerol hydrolase antibody
- AA673485 antibody
ab74681 at 1/500-1/1000 dilution staining ABHD6 in A549 cells by Immunofluorescence, in the absence (-) or presence (+) of the immunising peptide.
All lanes : Anti-ABHD6 antibody (ab74681) at 1/500 dilution
Lane 1 : COLO cell extracts
Lane 2 : COLO cell extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
Observed band size: 39 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab74681 has not yet been referenced specifically in any publications.