Overview

  • Product name

  • Description

    Rabbit polyclonal to ABHD6
  • Host species

    Rabbit
  • Specificity

    ab74681 detects endogenous levels of total ABHD6 protein.
  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal sequence of human ABHD6.

  • Positive control

    • COLO cell extracts and A549 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab74681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

Target

  • Relevance

    ABHD6 belongs to the alpha/beta hydrolase superfamily. It is highly expressed in mammalian brain and catalyzes the hydrolysis of the endocannabinoid 2-arachidonoyl glycerol (2-AG).
  • Cellular localization

    Membrane; Single-pass type II membrane protein
  • Database links

  • Alternative names

    • 0610041D24Rik antibody
    • 2 arachidonoylglycerol hydrolase antibody
    • AA673485 antibody
    • Abhydrolase domain containing 6 antibody
    • Abhydrolase domain containing protein 6 antibody
    • AV065425 antibody
    • Llipase protein antibody
    • MGC94917 antibody
    see all

Images

  • ab74681 at 1/500-1/1000 dilution staining ABHD6 in A549 cells by Immunofluorescence, in the absence (-) or presence (+) of the immunising peptide.
  • All lanes : Anti-ABHD6 antibody (ab74681) at 1/500 dilution

    Lane 1 : COLO cell extracts
    Lane 2 : COLO cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 39 kDa
    Observed band size: 39 kDa

References

ab74681 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry
Sample
Rat Cultured Cells (TSA cells)
Permeabilization
Yes - Triton
Specification
TSA cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Aug 03 2017

Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Brain)
Specification
Brain

Abcam user community

Verified customer

Submitted Aug 03 2017

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