Key features and details
- Rabbit polyclonal to Abi-1
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-Abi-1 antibody
See all Abi-1 primary antibodies
DescriptionRabbit polyclonal to Abi-1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human, Pig
Predicted to work with: Mouse, Rat
Recombinant fragment corresponding to Human Abi-1 aa 1-182. (N-terminal His-tag and GST-tag; Expressed in E.coli).
MAELQMLLEEEIPSGKRALIESYQNLTRVADYCENNYIQATDKRKALEET KAYTTQSLASVAYQINALANNVLQLLDIQASQLRRMESSINHISQTVDIH KEKVARREIGILTTNKNTSRTHKIIAPANMERPVRYIRKPIDYTVLDDVG HGVKWLKAKHGNNQPARTGTLSRTNPPTQKPP
Database link: Q8IZP0
- IHC-P: Human stomach, prostate cancer, cerebrum, glioma, kidney and stomach cancer tissues. WB: Human lung lysate; Pig cerebrum, liver and small intestine lysates; Recombinant human Abi-1 protein.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab233412 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233412 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 55 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionMay act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level.
Tissue specificityWidely expressed, with highest expression in brain.
Involvement in diseaseNote=A chromosomal aberration involving ABI1 is a cause of acute leukemias. Translocation t(10;11)(p11.2;q23) with MLL. ABI1 isoform 2 was found to be present in acute leukemia MLL-ABI1 fusion transcript.
Sequence similaritiesBelongs to the ABI family.
Contains 1 SH3 domain.
Contains 1 t-SNARE coiled-coil homology domain.
DomainThe t-SNARE coiled-coil homology domain is necessary and sufficient for interaction with STX1A.
modificationsIn vitro substrate for v-Abl (By similarity). Phosphorylated on tyrosine residues after serum stimulation or induction by v-Abl.
Cellular localizationCytoplasm. Nucleus. Cell projection > lamellipodium. Cell projection > filopodium. Cell projection > growth cone. Cell junction > synapse > synaptosome. Cytoplasm > cytoskeleton. Localized to protruding lamellipodia and filopodia tips. Also localized to neuronal growth cones and synaptosomes.
- Information by UniProt
- Abelson interactor 1 antibody
- Abi-1 antibody
- Abi1 antibody
Paraffin-embedded human stomach tissue stained for Abi-1 using ab233412 at 30 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human prostate cancer tissue stained for Abi-1 using ab233412 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human cerebrum tissue stained for Abi-1 using ab233412 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human glioma tissue stained for Abi-1 using ab233412 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human kidney tissue stained for Abi-1 using ab233412 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human stomach cancer tissue stained for Abi-1 using ab233412 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-Abi-1 antibody (ab233412) at 2 µg/ml + Recombinant human Abi-1 protein
Predicted band size: 55 kDa
All lanes : Anti-Abi-1 antibody (ab233412) at 1 µg/ml
Lane 1 : Human lung tissue lysate
Lane 2 : Pig cerebrum tissue lysate
Lane 3 : Pig liver tissue lysate
Lane 4 : Pig small intestine tissue lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 55 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233412 has not yet been referenced specifically in any publications.