Anti-Abi-1 antibody (ab72318)
Key features and details
- Rabbit polyclonal to Abi-1
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Abi-1 antibody
See all Abi-1 primary antibodies -
Description
Rabbit polyclonal to Abi-1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus -
Immunogen
Synthetic peptide within Human Abi-1 aa 325-375. The exact sequence is proprietary.
Database link: NP_005461.2 -
Positive control
- Whole cell lysates from HeLa and 293T cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/2000 - 1/10000. Detects a band of approximately 55, 60 kDa (predicted molecular weight: 55 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/2000 - 1/10000. Detects a band of approximately 55, 60 kDa (predicted molecular weight: 55 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level. -
Tissue specificity
Widely expressed, with highest expression in brain. -
Involvement in disease
Note=A chromosomal aberration involving ABI1 is a cause of acute leukemias. Translocation t(10;11)(p11.2;q23) with MLL. ABI1 isoform 2 was found to be present in acute leukemia MLL-ABI1 fusion transcript. -
Sequence similarities
Belongs to the ABI family.
Contains 1 SH3 domain.
Contains 1 t-SNARE coiled-coil homology domain. -
Domain
The t-SNARE coiled-coil homology domain is necessary and sufficient for interaction with STX1A. -
Post-translational
modificationsIn vitro substrate for v-Abl (By similarity). Phosphorylated on tyrosine residues after serum stimulation or induction by v-Abl. -
Cellular localization
Cytoplasm. Nucleus. Cell projection > lamellipodium. Cell projection > filopodium. Cell projection > growth cone. Cell junction > synapse > synaptosome. Cytoplasm > cytoskeleton. Localized to protruding lamellipodia and filopodia tips. Also localized to neuronal growth cones and synaptosomes. - Information by UniProt
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Database links
- Entrez Gene: 10006 Human
- Entrez Gene: 11308 Mouse
- Entrez Gene: 100738420 Pig
- Entrez Gene: 79249 Rat
- Omim: 603050 Human
- SwissProt: Q8IZP0 Human
- SwissProt: Q8CBW3 Mouse
- SwissProt: Q9QZM5 Rat
see all -
Alternative names
- Abelson interactor 1 antibody
- Abi-1 antibody
- Abi1 antibody
see all
Images
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All lanes : Anti-Abi-1 antibody (ab72318) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 55 kDa
Observed band size: 55,60 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 90 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling Abi-1 with ab72318 at 1/200 (1µg/ml). Detection: DAB.
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Detection of Human Abi-1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Lane 1: ab72317 used for IP at 3 µg/mg lysate. Lane 2: Abi-1 immunoprecipitated with an other antibody. Lane 3: ab72318 at 3 µg/mg lysate used for IP of Abi-1, which recognize downstream epitopes. Lane 4: control Ig IP For blotting immunoprecipitated Abi-1, ab72318 was used at 1 µg/ml.
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Detection of Abi-1 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab72318 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab72318 has not yet been referenced specifically in any publications.