Anti-ABI2 antibody (ab238832)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ABI2 antibody
See all ABI2 primary antibodies -
Description
Rabbit polyclonal to ABI2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human ABI2 aa 164-452.
Database link: Q9NYB9 -
Positive control
- IHC-P: Human brain and testis tissues. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity greater than 95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab238832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/50 - 1/200. | |
| IHC-P | 1/20 - 1/200. |
Target
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Function
May act in regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Regulates ABL1/c-Abl-mediated phosphorylation of MENA. -
Sequence similarities
Belongs to the ABI family.
Contains 1 SH3 domain.
Contains 1 t-SNARE coiled-coil homology domain. -
Post-translational
modificationsIs a substrate for ABL1. -
Cellular localization
Cytoplasm and Cell projection > lamellipodium. Cell projection > filopodium. Cytoplasm > cytoskeleton. Isoform 1 but not isoform 3 is localized to protruding lamellipodia and filopodia tips. - Information by UniProt
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Database links
- Entrez Gene: 10152 Human
- Omim: 606442 Human
- SwissProt: Q9NYB9 Human
- Unigene: 471156 Human
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Alternative names
- Abelson interactor 2 antibody
- ABI 2 antibody
- Abi-2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ABI2 antibody (ab238832)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ABI2 (green) using ab238832 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABI2 antibody (ab238832)Paraffin-embedded human brain tissue stained for ABI2 using ab238832 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABI2 antibody (ab238832)Paraffin-embedded human testis tissue stained for ABI2 using ab238832 at 1/100 dilution in immunohistochemical analysis.
References
ab238832 has not yet been referenced specifically in any publications.
