Product nameAnti-ABI3BP antibody
See all ABI3BP primary antibodies
DescriptionRabbit polyclonal to ABI3BP
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog
Synthetic peptide corresponding to a region within N terminal amino acids 143-192 (LINPHHDWTL PSHCPNDRFY TIRYREKDKE KKWIFQICPA TETIVENLKP) of Human ABI3BP (NP_056244)
- HeLa cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab82802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 119 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/62500.
Tissue specificityExpressed in brain, heart, lung, liver, pancreas kidney and placenta.
Sequence similaritiesContains 2 fibronectin type-III domains.
- Information by UniProt
- ABI gene family member 3 (NESH) binding protein antibody
- ABI gene family member 3 binding protein antibody
- ABI gene family member 3-binding protein antibody
Anti-ABI3BP antibody (ab82802) at 1 µg/ml + HeLa cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 119 kDa
Observed band size: 145 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa, 35 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 6-18%
ab82802 has not yet been referenced specifically in any publications.