ELISA abreview for Anti-ABL1 antibody

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Abreviews
Application
ELISA
Sample
Human Cell (K562 Cell Lysate)
Specification
K562 Cell Lysate
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Type
Sandwich (Detection)

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: Blocking buffer (5% BSA)
Dilution
1/10000

Secondary antibody

Secondary antibody
None used

Additional data

Additional Notes
Sandwich ELISA Step 1 Capture Antibody - c-Abl antibody [8E9] (ab3227) 1µg/ml in carbonate coating buffer Incubation time - 16 hrs @ 4°C Step 2 Blocking Buffer - 5% BSA in 1xPBS Incubation time - 16 hrs @ 4°C Step 3 Antigen - K562 cell lysate 1000ng/well Incubation time - 2 hrs @ room temperature Step 4 Detection Antibody - c-Abl antibody (ab47410) 1/10,000 in 1xPBS Incubation time - 2 hrs @ room temperature Step 5 Secondary Antibody - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) 1/10,000 Incubation time - 2 hrs @ room temperature

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Submitted Dec 01 2010

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