Anti-ABL1 (phospho Y245) antibody (ab193223)
Key features and details
- Rabbit polyclonal to ABL1 (phospho Y245)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ABL1 (phospho Y245) antibody
See all ABL1 primary antibodies -
Description
Rabbit polyclonal to ABL1 (phospho Y245) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human ABL1 (phospho Y245) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary. NCBI Protein#: NP_005148.2
Database link: P00519-2 -
Positive control
- Insulin treated K562 tissue extract.
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General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab193223 was purified by affinity-chromatography using an-epitope specific phosphopeptide. Non-phosphopeptide specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab193223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 123 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 123 kDa. |
Target
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Function
Protein kinase that regulates key processes linked to cell growth and survival. Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks. -
Tissue specificity
Widely expressed. -
Involvement in disease
Note=A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
Post-translational
modificationsPhosphorylated by PRKDC (By similarity). DNA damage-induced activation of c-Abl requires the function of ATM and Ser-446 phosphorylation (By similarity). Phosphorylation on Thr-735 is required for binding 14-3-3 proteins for cytoplasmic translocation.
Isoform IB is myristoylated on Gly-2. -
Cellular localization
Cytoplasm > cytoskeleton. Nucleus. Sequestered into the cytoplasm through interaction with 14-3-3 proteins and Nucleus membrane. The myristoylated c-ABL protein is reported to be nuclear. - Information by UniProt
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Database links
- Entrez Gene: 25 Human
- Entrez Gene: 11350 Mouse
- Entrez Gene: 311860 Rat
- Omim: 189980 Human
- SwissProt: P00519 Human
- SwissProt: P00520 Mouse
- Unigene: 431048 Human
- Unigene: 1318 Mouse
see all -
Alternative names
- Abelson murine leukemia viral oncogene homolog 1 antibody
- Abelson tyrosine protein kinase 1 antibody
- Abl 1 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab193223 has been referenced in 1 publication.
- Mohamed MF et al. CrkII/Abl phosphorylation cascade is critical for NLRC4 inflammasome activity and is blocked by Pseudomonas aeruginosa ExoT. Nat Commun 13:1295 (2022). PubMed: 35277504