Product nameAnti-ABL1 (phospho Y412) antibody
See all ABL1 primary antibodies
DescriptionRabbit polyclonal to ABL1 (phospho Y412)
Specificityab55284 detects endogenous levels of Abl1 only when phosphorylated at tyrosine 412.
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: African green monkey
Predicted to work with: Mouse, Rat, Human
Synthetic peptide corresponding to Human ABL1.
- extracts from COS7 cells, treated with Adriamycin
Previously labelled as c Abl.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab55284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Detects a band of approximately 123 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionProtein kinase that regulates key processes linked to cell growth and survival. Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks.
Tissue specificityWidely expressed.
Involvement in diseaseNote=A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
modificationsPhosphorylated by PRKDC (By similarity). DNA damage-induced activation of c-Abl requires the function of ATM and Ser-446 phosphorylation (By similarity). Phosphorylation on Thr-735 is required for binding 14-3-3 proteins for cytoplasmic translocation.
Isoform IB is myristoylated on Gly-2.
Cellular localizationCytoplasm > cytoskeleton. Nucleus. Sequestered into the cytoplasm through interaction with 14-3-3 proteins and Nucleus membrane. The myristoylated c-ABL protein is reported to be nuclear.
- Information by UniProt
- Abelson murine leukemia viral oncogene homolog 1 antibody
- Abelson tyrosine protein kinase 1 antibody
- Abl 1 antibody
All lanes : Anti-ABL1 (phospho Y412) antibody (ab55284) at 1/500 dilution
Lane 1 : extracts from COS7 cells, treated
with Adriamycin (0.5ng/ml, 24 hours).
Lane 2 : extracts from COS7 cells, treated
with Adriamycin (0.5ng/ml, 24 hours); ab55284 pre-incubated with the immunising phosphopeptide.
Lysates/proteins at 5 µg per lane.
All lanes : Goat Anti-Rabbit IgG (H+L)
Predicted band size: 123 kDa
Observed band size: 123 kDa
ab55284 has not yet been referenced specifically in any publications.