RecombinantRabMAb

Recombinant Anti-ABL2 antibody [EPR1222(2)] (ab134134)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1222(2)] to ABL2
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ABL2 antibody [EPR1222(2)]
    See all ABL2 primary antibodies

  • Description

    Rabbit monoclonal [EPR1222(2)] to ABL2

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 85% identity with ABL1. Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human ABL2 aa 500-600. The exact sequence is proprietary.

  • Positive control

    • Jurkat cell lysates; HeLa cell lysates; K562 cell lysates; Molt 4 cell lysates; RAW 264.7 cell lysates; PC 12 cell lysates; NIH 3T3 cell lysates

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab134134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 128 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.

    • Target

    • Function

      Non-receptor tyrosine-protein kinase that plays an ABL1-overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion and receptor endocytosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement); CTTN (involved in signaling); or TUBA1 and TUBB (microtubule subunits). Binds directly F-actin and regulates actin cytoskeletal structure through its F-actin-bundling activity. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL, DOK1 or ARHGAP35. Adhesion-dependent phosphorylation of ARHGAP35 promotes its association with RASA1, resulting in recruitment of ARHGAP35 to the cell periphery where it inhibits RHO. Phosphorylates multiple receptor tyrosine kinases like PDGFRB and other substrates which are involved in endocytosis regulation such as RIN1. In brain, may regulate neurotransmission by phosphorylating proteins at the synapse. ABL2 acts also as a regulator of multiple pathological signaling cascades during infection. Pathogens can highjack ABL2 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1.

    • Tissue specificity

      Widely expressed.

    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.

    • Domain

      Contains two distinct classes of F-actin-binding domains. Although both can bind F-actin, the 2 are required to bundle actin filaments.

    • Post-translational
      modifications

      Phosphorylated at Tyr-261 by ABL1 in response to oxidative stress. Phosphorylated by PDGFRB.
      Polyubiquitinated. Polyubiquitination of ABL2 leads to degradation.

    • Cellular localization

      Cytoplasm, cytoskeleton.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Abelson murine leukemia viral oncogene homolog 2 antibody
      • Abelson related gene protein antibody
      • Abelson tyrosine-protein kinase 2 antibody
      • Abelson-related gene protein antibody
      • ABL2 antibody
      • ABL2_HUMAN antibody
      • ABLL antibody
      • ARG antibody
      • Tyrosine kinase ARG antibody
      • Tyrosine protein kinase ABL2 antibody
      • Tyrosine-protein kinase ARG antibody
      • v abl Abelson murine leukemia viral oncogene homolog 2 antibody
      see all

    Images

    • Western blot - Anti-ABL2 antibody [EPR1222(2)] (ab134134)
      Western blot - Anti-ABL2 antibody [EPR1222(2)] (ab134134)
      All lanes : Anti-ABL2 antibody [EPR1222(2)] (ab134134) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : MOLT 4 cell lysate
      Lane 5 : RAW 264.7 cell lysate
      Lane 6 : PC12 cell lysate
      Lane 7 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size: 128 kDa

    • Flow Cytometry - Anti-ABL2 antibody [EPR1222(2)] (ab134134)
      Flow Cytometry - Anti-ABL2 antibody [EPR1222(2)] (ab134134)
      Flow cytometric analysis of permeabilized Jurkat cells labelling ABL2 (red) using ab134134 at 1/10 dilution or using a Rabbit IgG negative control (green)
    • OI-RD Scanning - Anti-ABL2 antibody [EPR1222(2)] (ab134134)
      OI-RD Scanning - Anti-ABL2 antibody [EPR1222(2)] (ab134134)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-ABL2 antibody [EPR1222(2)] (ab134134)
      Anti-ABL2 antibody [EPR1222(2)] (ab134134)

    References (2)

    Publishing research using ab134134? Please let us know so that we can cite the reference in this datasheet.

    ab134134 has been referenced in 2 publications.

    • Klein LE  et al. A humanin analog decreases oxidative stress and preserves mitochondrial integrity in cardiac myoblasts. Biochem Biophys Res Commun 440:197-203 (2013). WB . PubMed: 23985350
    • Hutterer C  et al. Profiling of the kinome of cytomegalovirus-infected cells reveals the functional importance of host kinases Aurora A, ABL and AMPK. Antiviral Res 99:139-48 (2013). WB ; Human . PubMed: 23648710

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