Key features and details
- Rabbit polyclonal to ABL2 - N-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ABL2 antibody - N-terminal
See all ABL2 primary antibodies
DescriptionRabbit polyclonal to ABL2 - N-terminal
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human ABL2 (N terminal). NP_001129472.
Database link: P42684
- HeLa and Jurkat cell lysate. Human liver cancer tissue.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab198718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/25 - 1/100.|
|WB||1/500 - 1/2000. Predicted molecular weight: 116 kDa.|
FunctionNon-receptor tyrosine-protein kinase that plays an ABL1-overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion and receptor endocytosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement); CTTN (involved in signaling); or TUBA1 and TUBB (microtubule subunits). Binds directly F-actin and regulates actin cytoskeletal structure through its F-actin-bundling activity. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL, DOK1 or ARHGAP35. Adhesion-dependent phosphorylation of ARHGAP35 promotes its association with RASA1, resulting in recruitment of ARHGAP35 to the cell periphery where it inhibits RHO. Phosphorylates multiple receptor tyrosine kinases like PDGFRB and other substrates which are involved in endocytosis regulation such as RIN1. In brain, may regulate neurotransmission by phosphorylating proteins at the synapse. ABL2 acts also as a regulator of multiple pathological signaling cascades during infection. Pathogens can highjack ABL2 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
DomainContains two distinct classes of F-actin-binding domains. Although both can bind F-actin, the 2 are required to bundle actin filaments.
modificationsPhosphorylated at Tyr-261 by ABL1 in response to oxidative stress. Phosphorylated by PDGFRB.
Polyubiquitinated. Polyubiquitination of ABL2 leads to degradation.
Cellular localizationCytoplasm, cytoskeleton.
- Information by UniProt
- Abelson murine leukemia viral oncogene homolog 2 antibody
- Abelson related gene protein antibody
- Abelson tyrosine-protein kinase 2 antibody
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling ABL2 with ab198718 at 1/25 dilution.
All lanes : Anti-ABL2 antibody - N-terminal (ab198718) at 1/400 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 116 kDa
Exposure time: 2 minutes
Gel: 8% SDS-PAGE
ab198718 has not yet been referenced specifically in any publications.