Key features and details
- Rabbit polyclonal to ABLIM1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ABLIM1 antibody
See all ABLIM1 primary antibodies
DescriptionRabbit polyclonal to ABLIM1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human ABLIM1 aa 174-319.
Database link: O14639
- WB: HeLa (human epithelial cell line from cervix adenocarcinoma) and HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysates. ICC/IF: HepG2 (human liver hepatocellular carcinoma cell line) cells. IHC-P: Human heart tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab222824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 88 kDa (predicted molecular weight: 87.7 kDa).|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionMay act as scaffold protein (By similarity). May play a role in the development of the retina. Has been suggested to play a role in axon guidance.
Tissue specificityDetected in liver, heart, skeletal muscle, brain and retina, where it is concentrated in the inner segment and in the outer plexiform layers.
Sequence similaritiesContains 1 HP (headpiece) domain.
Contains 4 LIM zinc-binding domains.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Associated with the cytoskeleton.
- Information by UniProt
- ABLIM 1 antibody
- ABLIM antibody
- abLIM-1 antibody
All lanes : Anti-ABLIM1 antibody (ab222824) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : Mouse heart lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 87.7 kDa
Observed band size: 88 kDa why is the actual band size different from the predicted?
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for ABLIM1 (green) using ab222824 at a dilution of 1/100 in ICC/IF. Secondary: Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Paraffin embedded sections of human heart tissue were stained for ABLIM1 with ab222824 at a dilution of 1/100.
ab222824 has not yet been referenced specifically in any publications.