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Rabbit polyclonal to ABLIM1
Predicted to work with:
Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan
Synthetic peptide, corresponding to a region between residue 50 and 100 of human ABLIM1 (NP_002304.3)
HeLa and 293T whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration information loading...
Immunogen affinity purified
ab85808 was affinity purified using an epitope specific to ABLIM1 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
Use at 5-10 µg/mg of lysate.
May act as scaffold protein (By similarity). May play a role in the development of the retina. Has been suggested to play a role in axon guidance.
Detected in liver, heart, skeletal muscle, brain and retina, where it is concentrated in the inner segment and in the outer plexiform layers.
Contains 1 HP (headpiece) domain.
Contains 4 LIM zinc-binding domains.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cytoplasm. Cytoplasm > cytoskeleton. Associated with the cytoskeleton.
Information by UniProt
ABLIM 1 antibody
Western blot - Anti-ABLIM1 antibody (ab85808)
All lanes : Anti-ABLIM1 antibody (ab85808) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Predicted band size: 88 kDa Observed band size: 88 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-ABLIM1 antibody (ab85808)
Detection of ABLIM1 by Western Blot of Immunprecipitate.
ab85808 at 1µg/ml staining ABLIM1 in HeLa whole cell lysate immunoprecipitated using ab85808 at 10µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
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