Overview

  • Product name

  • Description

    Rabbit polyclonal to ABLIM3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Human, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human ABLIM3 aa 550-650 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab156176)

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Mouse Embryonic Brain E16; Mouse Olfactory Bulb; Mouse Hippocampus; Mouse Cerebellum.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab129480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa). Abcam recommends using milk as the blocking agent - 3%

Target

Images

  • All lanes : Anti-ABLIM3 antibody (ab129480) at 1 µg/ml (Milk blocking - 3%)

    Lane 1 : E16 Ms Embryo Brain Tissue Lysate
    Lane 2 : Mouse Olfactory Bulb Lysate
    Lane 3 : Mouse Hippocampus Tissue Lysate
    Lane 4 : Cerebellum Mouse Tissue Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 78 kDa
    Observed band size: 78 kDa
    Additional bands at: 68 kDa, 70 kDa, 76 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    Abcam recommends using milk as the blocking agent - 3%. This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab129480 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

This product has been referenced in:

  • Guo N  et al. Dentate granule cell recruitment of feedforward inhibition governs engram maintenance and remote memory generalization. Nat Med 24:438-449 (2018). Read more (PubMed: 29529016) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab129480.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up