Anti-ABO antibody [BRIC231] (ab33404)

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Overview

  • Product name

    Anti-ABO antibody [BRIC231]

  • Description

    Mouse monoclonal [BRIC231] to ABO

  • Host species

    Mouse

  • Tested applications

    Suitable for: Agglutination, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus corresponding to Human ABO.

Properties

Applications

Our Abpromise guarantee covers the use of ab33404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Agglutination Use at an assay dependent concentration.
Flow Cyt Use 10µl for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity.

  • Pathway

    Protein modification; protein glycosylation.

  • Sequence similarities

    Belongs to the glycosyltransferase 6 family.

  • Domain

    The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.

  • Post-translational
    modifications

    The soluble form derives from the membrane form by proteolytic processing.

  • Cellular localization

    Golgi apparatus > Golgi stack membrane. Secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
  • Information by UniProt

  • Database links

    • Alternative names

      • A transferase antibody
      • Abo antibody
      • B transferase antibody
      • BGAT_HUMAN antibody
      • Blood Group H2 antigen antibody
      • CD173 antibody
      • Fucosylglycoprotein 3-alpha-galactosyltransferase antibody
      • Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase antibody
      • Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form antibody
      • Glycoprotein-fucosylgalactoside alpha-galactosyltransferase antibody
      • Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase antibody
      • Histo-blood group A transferase antibody
      • Histo-blood group B transferase antibody
      • NAGAT antibody
      see all

    References

    This product has been referenced in:

    • Purohit S  et al. Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. Nat Commun 9:258 (2018). Read more (PubMed: 29343722) »
    • Gao C  et al. Carbohydrate Sequence of the Prostate Cancer-associated Antigen F77 Assigned by a Mucin O-Glycome Designer Array. J Biol Chem 289:16462-77 (2014). Read more (PubMed: 24753245) »
    See all 2 Publications for this product

    Publishing research using ab33404? Please let us know so that we can cite the reference in this datasheet.

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