Anti-ABO antibody [BRIC231] (ab33404)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-ABO antibody [BRIC231]
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DescriptionMouse monoclonal [BRIC231] to ABO
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Host speciesMouse
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Tested applicationsSuitable for: Agglutination, Flow Cytmore details
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Species reactivityReacts with: Human
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Immunogen
Tissue, cells or virus corresponding to Human ABO.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: Tris buffered glycine. pH 7.4 -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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Clone numberBRIC231
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab33404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Agglutination | Use at an assay dependent concentration. | |
| Flow Cyt | Use 10µl for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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FunctionThis protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity.
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PathwayProtein modification; protein glycosylation.
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Sequence similaritiesBelongs to the glycosyltransferase 6 family.
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DomainThe conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.
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Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing. -
Cellular localizationGolgi apparatus > Golgi stack membrane. Secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
- Information by UniProt
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Database links
- Entrez Gene: 28 Human
- Omim: 110300 Human
- SwissProt: P16442 Human
- Unigene: 654423 Human
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Alternative names
- A transferase antibody
- Abo antibody
- B transferase antibody
see all
Protocols
References
This product has been referenced in:
- Purohit S et al. Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. Nat Commun 9:258 (2018). Read more (PubMed: 29343722) »
- Gao C et al. Carbohydrate Sequence of the Prostate Cancer-associated Antigen F77 Assigned by a Mucin O-Glycome Designer Array. J Biol Chem 289:16462-77 (2014). Read more (PubMed: 24753245) »
