Overview

  • Product name
    ABTS™, Peroxidase substrate
  • Description
    Peroxidase substrate
  • Biological description
    Peroxidase substrate suitable for ELISA applications. Soluble end product can be read using spectrophotometry (405 nm).
  • CAS Number
    28752-68-3
  • Chemical structure
    Chemical Structure

Properties

  • Chemical name

    3-Ethyl-2-[(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid

  • Molecular weight
    514.60
  • Molecular formula
    C18H18N4O6S4
  • PubChem identifier
    35688
  • Storage instructions
    Store at +4°C.
  • Solubility overview

    Supplied in citrate buffer (pH 4; 1.46 mM)

  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES
    CCN1C2=C(C=C(C=C2)S(=O)(=O)O)SC1=NN=C3N(C4=C(S3)C=C(C=C4)S(=O)(=O)O)CC
  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:
  • Szutowicz A  et al. Colorimetric assay for monoamine oxidase in tissues using peroxidase and 2,2'-azinodi(3-ethylbenzthiazoline-6-sulfonic acid) as chromogen. Anal Biochem 138:86-94 (1984). Read more (PubMed: 6329037) »
  • Matsuda H  et al. Evaluation of ELISA with ABTS, 2-2'-azino-di-(3-ethylbenzthiazoline sulfonic acid), as the substrate of peroxidase and its application to the diagnosis of schistosomiasis. Jpn J Exp Med 54:131-8 (1984). Read more (PubMed: 6542945) »
  • Groome NP Superiority of ABTS over Trinder reagent as chromogen in highly sensitive peroxidase assays for enzyme linked immunoadsorbent assay. J Clin Chem Clin Biochem 18:345-9 (1980). Read more (PubMed: 6159451) »

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