Product nameAc-TVASSSTA Octapeptide peptide
Our Abpromise guarantee covers the use of ab95132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 2.38% HEPES, 0.58% Sodium chloride
ReconstitutionHydrate in 0.5ml of H2O.
RelevanceAc-TVASSSTA is an Octapeptide mimic of the N-terminal residues of the reactive center loop of PAI-1 (cf. P14-P7 residues). The Octapeptide inactivates PAI-1 by substituting for strand 4 in beta-sheet A in a manner that competes with formation of the latent species. Insertion of the peptide into beta-sheet A effectively forms a stable complex that converts PAI-1 into a substrate for tissue-type plasminogen activator (tPA) and other target proteinases. The resulting species of PAI-1 is thermodynamically stable and is useful for investigating the role of reactive center loop insertion.
Mass spectrometry analysis of ab95132
HPLC analysis of ab95132
10% SDS-PAGE Reduced
Lane 1: PAI-1 (5 ug) Frozen Lane 2: PAI-1 (5 ug) Incubated
Lane 3: PAI-1 (5 ug) + ab95132 (3 ug) Incubated Lane 4: PAI-1 (5 ug) Frozen + tPA (0.07 ug)
Lane 5: PAI-1 (5 ug) Incubated + tPA (0.07 ug) Lane 6: PAI-1 (5 ug) + ab95132 (3 ug) Incubated + tPA (0.07 ug)
Lane 7: Molecular weight markers
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab95132 has not yet been referenced specifically in any publications.