Overview

  • Product name

  • Description

    Rabbit polyclonal to AC5
  • Host species

    Rabbit
  • Specificity

    This antibody can cross-react with ADCY6.
  • Tested applications

    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human AC5 (internal sequence).

  • Positive control

    • Extracts from COLO205 cells.
  • General notes

    Previously labelled as ADCY5. 

Properties

Applications

Our Abpromise guarantee covers the use of ab66037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 139 kDa (predicted molecular weight: 139 kDa).
ELISA 1/10000.

Target

Images

  • All lanes : Anti-AC5 antibody (ab66037) at 1/500 dilution

    Lane 1 : Extracts from COLO205 cells with no immunising peptide
    Lane 2 : Extracts from COLO205 cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 139 kDa
    Observed band size: 139 kDa

  • ICC/IF image of ab66037 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66037, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

See all 3 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your inquiry.


The epitope is at a cytoplasmic location, and the immunogen sequence falls within amino acids 1000 to 1100.


We do not currently have the blocking peptide in stock, however I can get it added to the catalogue if you'd like. But we do have HeLa cell lysate in the catalogue as ab29545 that you can use in WB.


https://www.abcam.com/HeLa-Human-epithelial-carcinoma-cell-line-Whole-Cell-Lysate-ab29545.html


I hope this information helps and let me know if you are interested in having the blocking peptide added, or if you are satisfied with the HeLa lysate.

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