Overview

  • Product name
    Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7]
    See all ACAA1/Beta-ketothiolase primary antibodies
  • Description
    Mouse monoclonal [2H6BE7] to ACAA1/Beta-ketothiolase
  • Host species
    Mouse
  • Tested applications
    Suitable for: ICC, Flow Cyt, IP, WB, In-Cell ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Tissue, cells or virus corresponding to Human ACAA1/Beta-ketothiolase.

  • Positive control
    • HepG2,HL-60 cells, human liver tissue, rat liver tissue and mouse liver tissue.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

     This product was previously labelled as ACAA1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab110289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 5 µg/ml. Heat-induced antigen retrieval improves signal
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IP Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa.
In-Cell ELISA Use a concentration of 1 µg/ml.

Target

Images

  • All lanes : Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289) at 1 µg/ml

    Lane 1 : Human liver
    Lane 2 : HepG2
    Lane 3 : Rat liver
    Lane 4 : Mouse liver

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 44 kDa

  • ACAA1/Beta-ketothiolase was immunoprecipitated using 0.5mg Mouse Liver tissue lysate, 5µg of Mouse monoclonal to ACAA1/Beta-ketothiolase and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab110289.

    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.

    Band: 44kDa; ACAA1/Beta-ketothiolase

  • ab110289, at 5 µg/mL, staining ACAA1/Beta-ketothiolase in Human HepG2 cells. DAPI was used to stain the cell nuclei (blue).

  • ab110289, at 1 µg/mL, staining ACAA1/Beta-ketothiolase in HL-60 cells (blue) or using an isotype control (red).

References

This product has been referenced in:
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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