Anti-ACAA1/Beta-ketothiolase antibody (ab228747)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-ACAA1/Beta-ketothiolase antibody
See all ACAA1/Beta-ketothiolase primary antibodies -
Description
Rabbit polyclonal to ACAA1/Beta-ketothiolase -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow
-
Immunogen
Recombinant fragment within Human ACAA1/Beta-ketothiolase (internal sequence). The exact sequence is proprietary.
Database link: P09110 -
Positive control
- WB: HepG2 whole cell lysate. ICC/IF: HeLa and HepG2 cells.
-
General notes
This product was previously labelled as ACAA1
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab228747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 44 kDa. | |
| ICC/IF | 1/100 - 1/1000. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Pathway
Lipid metabolism; fatty acid metabolism. -
Sequence similarities
Belongs to the thiolase family. -
Cellular localization
Peroxisome. - Information by UniProt
-
Database links
- Entrez Gene: 508324 Cow
- Entrez Gene: 30 Human
- Entrez Gene: 113868 Mouse
- Omim: 604054 Human
- SwissProt: P09110 Human
- SwissProt: Q921H8 Mouse
- Unigene: 643487 Human
- Unigene: 205266 Mouse
-
Alternative names
- 3 ketoacyl CoA thiolase peroxisomal antibody
- 3-ketoacyl-CoA thiolase antibody
- ACAA antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody (ab228747)Paraffin-embeded mouse liver tissue labeling ACAA1/Beta-ketothiolase using ab228747 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 minutes.
-
Immunocytochemistry/ Immunofluorescence - Anti-ACAA1/Beta-ketothiolase antibody (ab228747)Ice-cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ACAA1/Beta-ketothiolase (green) using ab228747 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
-
Western blot - Anti-ACAA1/Beta-ketothiolase antibody (ab228747)Anti-ACAA1/Beta-ketothiolase antibody (ab228747) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 44 kDa10% SDS-PAGE gel.
-
Immunocytochemistry/ Immunofluorescence - Anti-ACAA1/Beta-ketothiolase antibody (ab228747)Methanol-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for ACAA1/Beta-ketothiolase (green) using ab228747 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
References
ab228747 has not yet been referenced specifically in any publications.
