Anti-ACAA1/Beta-ketothiolase antibody (ab228747)
Key features and details
- Rabbit polyclonal to ACAA1/Beta-ketothiolase
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ACAA1/Beta-ketothiolase antibody
See all ACAA1/Beta-ketothiolase primary antibodies -
Description
Rabbit polyclonal to ACAA1/Beta-ketothiolase -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment within Human ACAA1/Beta-ketothiolase (internal sequence). The exact sequence is proprietary.
Database link: P09110 -
Positive control
- WB: HepG2 whole cell lysate. ICC/IF: HeLa and HepG2 cells.
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General notes
This product was previously labelled as ACAA1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab228747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 44 kDa. | |
| ICC/IF | 1/100 - 1/1000. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Pathway
Lipid metabolism; fatty acid metabolism. -
Sequence similarities
Belongs to the thiolase family. -
Cellular localization
Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 508324 Cow
- Entrez Gene: 30 Human
- Entrez Gene: 113868 Mouse
- Omim: 604054 Human
- SwissProt: P09110 Human
- SwissProt: Q921H8 Mouse
- Unigene: 643487 Human
- Unigene: 205266 Mouse
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Alternative names
- 3 ketoacyl CoA thiolase peroxisomal antibody
- 3-ketoacyl-CoA thiolase antibody
- ACAA antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody (ab228747)
Paraffin-embeded mouse liver tissue labeling ACAA1/Beta-ketothiolase using ab228747 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 minutes.
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Immunocytochemistry/ Immunofluorescence - Anti-ACAA1/Beta-ketothiolase antibody (ab228747)Ice-cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ACAA1/Beta-ketothiolase (green) using ab228747 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
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Western blot - Anti-ACAA1/Beta-ketothiolase antibody (ab228747)Anti-ACAA1/Beta-ketothiolase antibody (ab228747) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 44 kDa10% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-ACAA1/Beta-ketothiolase antibody (ab228747)Methanol-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for ACAA1/Beta-ketothiolase (green) using ab228747 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab228747 has not yet been referenced specifically in any publications.
