Recombinant Anti-ACAA2 antibody [EPR6732(2)] (ab128929)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6732(2)] to ACAA2
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ACAA2 antibody [EPR6732(2)]
See all ACAA2 primary antibodies -
Description
Rabbit monoclonal [EPR6732(2)] to ACAA2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, Caco-2 or HepG2 lysate, IHC-P: Human kidney tissue, ICC: HepG2 cells. Flow Cyt (intra): RPMI-8226 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Dissociation constant (KD)
KD = 7.10 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6732(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Use of an HRP/AP polymerized secondary antibody recommended. |
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ICC/IF |
1/250 - 1/500.
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Notes |
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Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Use of an HRP/AP polymerized secondary antibody recommended. |
ICC/IF
1/250 - 1/500. |
Target
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Function
Abolishes BNIP3-mediated apoptosis and mitochondrial damage. -
Pathway
Lipid metabolism; fatty acid metabolism. -
Sequence similarities
Belongs to the thiolase family. -
Cellular localization
Mitochondrion. Colocalizes with BNIP3 in the mitochondria. - Information by UniProt
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Database links
- Entrez Gene: 10449 Human
- Omim: 604770 Human
- SwissProt: P42765 Human
- Unigene: 200136 Human
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Alternative names
- 3 ketoacyl CoA thiolase antibody
- 3 ketoacyl CoA thiolase mitochondrial antibody
- 3-ketoacyl-CoA thiolase, mitochondrial antibody
see all
Images
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All lanes : Anti-ACAA2 antibody [EPR6732(2)] (ab128929) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Caco-2 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 42 kDa -
ab128929, at a 1/50 dilution, staining Human ACAA2 in kidney, using Immunohistochemistry, Formalin/PFA-fixed paraffin-emebedded tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab128929, at a 1/250 dilution, staining Human ACAA2 in HepG2 cells, using Immunofluorescence.
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Flow Cytometry analysis of RPMI-8226 (Human plasmacytoma, myeloma B Lymphocyte) cells labeling ACAA2 with purified ab128929 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab128929 has been referenced in 1 publication.
- Zhang YK et al. Enoyl-CoA hydratase-1 regulates mTOR signaling and apoptosis by sensing nutrients. Nat Commun 8:464 (2017). PubMed: 28878358