Overview

  • Product name

    Anti-ACAA2 antibody [EPR6733]
    See all ACAA2 primary antibodies
  • Description

    Rabbit monoclonal [EPR6733] to ACAA2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ACAA2 aa 350-450. The exact sequence is proprietary.

  • Positive control

    • HepG2, 293T, and HeLa whole cell lysate (ab150035); Human colonic carcinoma tissue. IF/ICC: HepG2 cell line.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab128911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Abolishes BNIP3-mediated apoptosis and mitochondrial damage.
    • Pathway

      Lipid metabolism; fatty acid metabolism.
    • Sequence similarities

      Belongs to the thiolase family.
    • Cellular localization

      Mitochondrion. Colocalizes with BNIP3 in the mitochondria.
    • Information by UniProt
    • Database links

    • Alternative names

      • 3 ketoacyl CoA thiolase antibody
      • 3 ketoacyl CoA thiolase mitochondrial antibody
      • 3-ketoacyl-CoA thiolase, mitochondrial antibody
      • 3-oxoacyl-CoA thiolase, mitochondrial antibody
      • ACAA 2 antibody
      • Acaa2 antibody
      • Acetyl CoA acyltransferase 2 antibody
      • Acetyl CoA acyltransferase antibody
      • Acetyl Coenzyme A acyltransferase 2 antibody
      • Acetyl-CoA acyltransferase antibody
      • Beta ketothiolase antibody
      • Beta-ketothiolase antibody
      • DSAEC antibody
      • Mitochondrial 3 oxoacyl CoA thiolase antibody
      • Mitochondrial 3 oxoacyl Coenzyme A thiolase antibody
      • Mitochondrial 3-oxoacyl-CoA thiolase antibody
      • T1 antibody
      • THIM_HUMAN antibody
      see all

    Images

    • All lanes : Anti-ACAA2 antibody [EPR6733] (ab128911) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 41 kDa

    • ab128911 stained HepG2 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab128911 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • ab128911 at 1/100 dilution staining ACAA2 in paraffin embedded Human colonic carcinoma tissue by immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab128911 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab128911.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up