Overview

  • Product name

  • Description

    Mouse polyclonal to ACAD9
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length ACAD9 (NP_054768.2) protein (Human)

  • Positive control

    • Human liver mitochondrial lysate and ACAD9 transfected 293T lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab72655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 69 kDa.

Target

  • Relevance

    ACAD9 belongs to a group of Acyl CoA dehydrogenases (ACADs). This family of proteins localize to the mitochondria and catalyze the rate-limiting step in the beta-oxidation of fatty acyl-CoA. One of the main energy producing metabolic pathways in eukaryotes is mitochondrial fatty acid beta oxidation. ACADs act on fatty acids containing 14 to 20 carbons. ACAD9 is specifically active toward palmitoyl-CoA and long-chain unsaturated substrates. Mutations in this gene cause acyl-CoA dehydrogenase family member type 9 deficiency. Alternate splicing results in multiple transcript variants.
  • Cellular localization

    Mitochondrial
  • Database links

  • Alternative names

    • Acyl CoA dehydrogenase 9 antibody
    • Acyl Coenzyme A dehydrogenase family, member 9 antibody
    • acyl-CoA dehydrogenase family member 9, mitochondrial antibody
    • FLJ23533 antibody
    • MGC14452 antibody
    • NPD002 antibody
    • Very long chain acyl CoA dehydrogenase VLCAD antibody
    see all

Images

  • Anti-ACAD9 antibody (ab72655) at 1/500 dilution + Human liver tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size: 69 kDa
    Observed band size: 59 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-ACAD9 antibody (ab72655) at 1/500 dilution

    Lane 1 : ACAD9 transfected 293T lysate
    Lane 2 : Non transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size: 69 kDa
    Observed band size: 59 kDa why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Wang M  et al. A Deafness- and Diabetes-associated tRNA Mutation Causes Deficient Pseudouridinylation at Position 55 in tRNAGlu and Mitochondrial Dysfunction. J Biol Chem 291:21029-21041 (2016). Read more (PubMed: 27519417) »
  • Wang M  et al. A deafness-associated tRNAAsp mutation alters the m1G37 modification, aminoacylation and stability of tRNAAsp and mitochondrial function. Nucleic Acids Res 44:10974-10985 (2016). Read more (PubMed: 27536005) »
See all 2 Publications for this product

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