Recombinant
RabMAb

Recombinant Anti-ACADL/LCAD antibody [EPR16341] (ab196655)

Overview

  • Product name

    Anti-ACADL/LCAD antibody [EPR16341]
    See all ACADL/LCAD primary antibodies
  • Description

    Rabbit monoclonal [EPR16341] to ACADL/LCAD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ACADL/LCAD aa 300-400. The exact sequence is proprietary.
    Database link: P28330

  • Positive control

    • 293, HeLa, HepG2, HCT116, C6, RAW 264.7, PC12 and NIH 3T3 cell lysates.
  • General notes

     

     

    Previously labelled as ACADL. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab196655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).

Target

  • Pathway

    Lipid metabolism; mitochondrial fatty acid beta-oxidation.
  • Involvement in disease

    Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD) [MIM:201475]. An inborn error of mitochondrial fatty acid beta-oxidation which leads to impaired long-chain fatty acid beta-oxidation. It is clinically heterogeneous, with three major phenotypes: a severe childhood form characterized by early onset, high mortality and high incidence of cardiomyopathy; a milder childhood form with later onset, characterized by hypoketotic hypoglycemia, low mortality and rare cardiomyopathy; an adult form, with isolated skeletal muscle involvement, rhabdomyolysis and myoglobinuria, usually triggered by exercise or fasting.
  • Sequence similarities

    Belongs to the acyl-CoA dehydrogenase family.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACAD4 antibody
    • ACADL antibody
    • ACADL_HUMAN antibody
    • Acyl Coenzyme A dehydrogenase long chain antibody
    • Acyl-CoA dehydrogenase long chain antibody
    • FLJ94052 antibody
    • LCAD antibody
    • Long chain acyl CoA dehydrogenase antibody
    • Long-chain specific acyl-CoA dehydrogenase, mitochondrial antibody
    see all

Images

  • All lanes : Anti-ACADL/LCAD antibody [EPR16341] (ab196655) at 1/2000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW 264.7 cell lysate
    Lane 3 : PC12 cell lysate
    Lane 4 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 48 kDa

  • All lanes : Anti-ACADL/LCAD antibody [EPR16341] (ab196655) at 1/10000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HCT116 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 48 kDa

References

This product has been referenced in:

  • Becker C  et al. CLPP deficiency protects against metabolic syndrome but hinders adaptive thermogenesis. EMBO Rep 19:N/A (2018). Read more (PubMed: 29588285) »
  • Brouwers B  et al. Elevated Nicotinamide Phosphoribosyl Transferase in Skeletal Muscle Augments Exercise Performance and Mitochondrial Respiratory Capacity Following Exercise Training. Front Physiol 9:704 (2018). Read more (PubMed: 29942262) »
See all 4 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (human hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
human hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Dec 28 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart (Embryonic))
Permeabilization
Yes - Tween-20
Specification
Heart (Embryonic)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 29 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 19 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up