Anti-ACADM antibody [EPR3708] (ab92461)


  • Product name
    Anti-ACADM antibody [EPR3708]
    See all ACADM primary antibodies
  • Description
    Rabbit monoclonal [EPR3708] to ACADM
  • Host species
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ACADM aa 200-300. The exact sequence is proprietary.

  • Positive control
    • WB: Human heart or fetal liver tissue lysates; HeLa, HepG2, or K562 cell lysates. IHC-P: Human liver tissue. ICC/IF: HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab92461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    ICC/IF: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended.
    IP: 1/10 - 1/100.
    WB: 1/10000 - 1/50000. Predicted molecular weight: 47 kDa.

    Is unsuitable for Flow Cyt.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      This enzyme is specific for acyl chain lengths of 4 to 16.
    • Pathway
      Lipid metabolism; mitochondrial fatty acid beta-oxidation.
    • Involvement in disease
      Defects in ACADM are the cause of acyl-CoA dehydrogenase medium-chain deficiency (ACADMD) [MIM:201450]. It is an autosomal recessive disease which causes fasting hypoglycemia, hepatic dysfunction, and encephalopathy, often resulting in death in infancy.
    • Sequence similarities
      Belongs to the acyl-CoA dehydrogenase family.
    • Cellular localization
      Mitochondrion matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACAD 1 antibody
      • ACAD1 antibody
      • Acadm antibody
      • ACADM_HUMAN antibody
      • Acyl coenzyme A dehydrogenase antibody
      • Acyl coenzyme A dehydrogenase C 4 to C 12 straight chain antibody
      • FLJ18227 antibody
      • FLJ93013 antibody
      • FLJ99884 antibody
      • MCAD antibody
      • MCADH antibody
      • Medium chain acyl CoA dehydrogenase antibody
      • Medium chain fatty acyl CoA dehydrogenase antibody
      • Medium chain specific acyl CoA dehydrogenase antibody
      • Medium chain specific acyl CoA dehydrogenase mitochondrial antibody
      • Medium-chain specific acyl-CoA dehydrogenase antibody
      • mitochondrial antibody
      see all


    • ab92461, at 1/100 dilution, staining ACADM in formalin-fixed, paraffin-embedded Human liver tissue by immunohistochemistry.
    • Immunofluorescent staining of ACADM in HeLa cells using ab92461 at 1/100 dilution.
    • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ACADM with unpurified ab92461 at 1/50 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    • All lanes : Anti-ACADM antibody [EPR3708] (ab92461) at 1/10000 dilution

      Lane 1 : Human heart lysate
      Lane 2 : fetal liver lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP conjugated Goat anti-Rabbit Ig at 1/2000 dilution

      Predicted band size: 47 kDa


    This product has been referenced in:
    • Stancic A  et al. A lesson from the oxidative metabolism of hibernator heart: Possible strategy for cardioprotection. Comp Biochem Physiol B Biochem Mol Biol 219-220:1-9 (2018). WB . Read more (PubMed: 29501789) »
    • Tan EP  et al. Altering O-linked ß-N-acetylglucosamine cycling disrupts mitochondrial function. J Biol Chem 289:14719-30 (2014). WB . Read more (PubMed: 24713701) »

    See all 4 Publications for this product

    Customer reviews and Q&As

    Western blot
    Human Cell lysate - whole cell (human hepatocytes)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    15 µg
    human hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Abcam user community

    Verified customer

    Submitted Dec 28 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Immunocytochemistry/ Immunofluorescence
    Human Cell (HEK293)
    Blocking step
    Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

    Abcam user community

    Verified customer

    Submitted Apr 03 2017

    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (10% SDS PAGE)
    Mouse Tissue lysate - whole (Brain)
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted May 27 2014

    I appreciate the time that has been spent on these experiments and would be pleased to arrange, in compensation, a replacement of ab92461 with another anti-ACADM such as ab110296. Alternatively I can also arrange a credit note. I look forward to hea...

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    Thank you very much for your email. My colleague is in contact with the laboratory in order to try to find out what the problem might is. Indeed we have to investigate this issue further as the quality of our products is most important. We have had no ...

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    Thank you for sending the image. We can’t make assumptions for the specificity of an antibody because of its same size band specificity of as of beta actin. Many protein have similar sizes, so experimental evidence is always good to prove the cross...

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    Thank you for contacting us. The antibody is specific against ACADM protein only; ab92461 should not recognize the beta actin protein. Could you please explain why you think the antibody is detecting the beta actin protein? In regards to the exp...

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    Thank you for your enquiry regarding ab92461 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. I appreciate the time you have spent on these experimen...

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