Key features and details
- Mouse monoclonal [3B7BH7] to ACADM/MCAD
- Suitable for: WB, Flow Cyt, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
Product nameAnti-ACADM/MCAD antibody [3B7BH7]
See all ACADM/MCAD primary antibodies
DescriptionMouse monoclonal [3B7BH7] to ACADM/MCAD
Tested applicationsSuitable for: WB, Flow Cyt, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human
Full length protein. This information is considered to be commercially sensitive.
- HeLa cells, HL-60 cells, Human cerebellum, HepG2 cells, HeLa cells, H9C2 (rat cells), and MEF (mouse cells) lysates.
This antibody clone is manufactured by Abcam.
MCAD (medium-chain acyl-CoA dehydrogenase) is an oxidoreductase enzyme of the mitochondrial fatty acid beta-oxidation pathway that is specific for acyl chain lengths of 4 to 16. It also utilizes the electron transfer flavoprotein (ETF) as electron acceptor that transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
Product was previously marketed under the MitoSciences sub-brand.
Previously labelled as ACADM.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99.98% HEPES buffered saline
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Purification notesProduced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Primary antibody notesMCAD (medium-chain acyl-CoA dehydrogenase) is an oxidoreductase enzyme of the mitochondrial fatty acid beta-oxidation pathway that is specific for acyl chain lengths of 4 to 16. It also utilizes the electron transfer flavoprotein (ETF) as electron acceptor that transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
Light chain typekappa
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab110296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.125 µg/ml. Predicted molecular weight: 47 kDa.|
|Flow Cyt||Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-P||1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionThis enzyme is specific for acyl chain lengths of 4 to 16.
PathwayLipid metabolism; mitochondrial fatty acid beta-oxidation.
Involvement in diseaseDefects in ACADM are the cause of acyl-CoA dehydrogenase medium-chain deficiency (ACADMD) [MIM:201450]. It is an autosomal recessive disease which causes fasting hypoglycemia, hepatic dysfunction, and encephalopathy, often resulting in death in infancy.
Sequence similaritiesBelongs to the acyl-CoA dehydrogenase family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- ACAD 1 antibody
- ACAD1 antibody
- Acadm antibody
All lanes : Anti-ACADM/MCAD antibody [3B7BH7] (ab110296) at 0.125 µg/ml
Lane 1 : HepG2 cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : H9C2 (rat cells) lysates
Lane 4 : MEF (mouse cells) lysates
Lysates/proteins at 15 µg per lane.
Predicted band size: 47 kDa
Immunocytochemistry image of ab110296 stained Human HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with ab110296 (1 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
ACADM/MCAD immunohistochemistry in Human cerebellum visualized with ab110296 at 1/1000. MCAD immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells. Note the distinctive subcellular localization of MCAD immunoreactivity in the Purkinje cell bodies.
HL-60 cells were stained with 1 µg/mL ab110296 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
HL-60 cells were incubated at 37°C for 24h with vehicle control (0 µM) and different concentrations of fenofibrate (ab120832). Increased expression of ACADM/MCAD in HL-60 cells correlates with an increase in fenofibrate concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab110296 at 1 µg/ml and ab9484 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 dilution and visualised using ECL development solution.
ab110296 has been referenced in 7 publications.
- Lim SC et al. Loss of the Mitochondrial Fatty Acid ß-Oxidation Protein Medium-Chain Acyl-Coenzyme A Dehydrogenase Disrupts Oxidative Phosphorylation Protein Complex Stability and Function. Sci Rep 8:153 (2018). WB ; Human . PubMed: 29317722
- Shaltouki A et al. Alpha-synuclein delays mitophagy and targeting Miro rescues neuron loss in Parkinson's models. Acta Neuropathol 136:607-620 (2018). PubMed: 29923074
- Cornelius N et al. Cellular consequences of oxidative stress in riboflavin responsive multiple acyl-CoA dehydrogenation deficiency patient fibroblasts. Hum Mol Genet N/A:N/A (2014). PubMed: 24698980
- Tomilov A et al. Shc depletion stimulates brown fat activity in vivo and in vitro. Aging Cell 13:1049-58 (2014). Mouse . PubMed: 25257068
- Peek CB et al. Circadian clock NAD+ cycle drives mitochondrial oxidative metabolism in mice. Science 342:1243417 (2013). PubMed: 24051248
- Tucci S et al. Tissue-Specific Strategies of the Very-Long Chain Acyl-CoA Dehydrogenase-Deficient (VLCAD(-/-)) Mouse to Compensate a Defective Fatty Acid ß-Oxidation. PLoS One 7:e45429 (2012). WB ; Mouse . PubMed: 23024820
- Frier BC et al. Interactions between the consumption of a high-fat diet and fasting in the regulation of fatty acid oxidation enzyme gene expression: an evaluation of potential mechanisms. Am J Physiol Regul Integr Comp Physiol 300:R212-21 (2011). PubMed: 21084676