Key features and details
- Goat polyclonal to ACADM/MCAD
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ACADM/MCAD antibody
See all ACADM/MCAD primary antibodies
DescriptionGoat polyclonal to ACADM/MCAD
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
- Human Heart Lysate
Previously labelled as ACADM.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab13677 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.01 - 0.1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).Can be blocked with Human ACADM/MCAD peptide (ab23229).
A 1 hour primary incubation is recommended for this product.
|IHC-P||Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionThis enzyme is specific for acyl chain lengths of 4 to 16.
PathwayLipid metabolism; mitochondrial fatty acid beta-oxidation.
Involvement in diseaseDefects in ACADM are the cause of acyl-CoA dehydrogenase medium-chain deficiency (ACADMD) [MIM:201450]. It is an autosomal recessive disease which causes fasting hypoglycemia, hepatic dysfunction, and encephalopathy, often resulting in death in infancy.
Sequence similaritiesBelongs to the acyl-CoA dehydrogenase family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- ACAD 1 antibody
- ACAD1 antibody
- Acadm antibody
ab13677 (3.75 µg/ml) staining ACADM/MCAD in paraffin-embedded human Adrenal Gland. Steamed antigen retieval with citrate buffer (pH 6) was carried out.
Anti-ACADM/MCAD antibody (ab13677) at 0.05 µg/ml + Human heart lysates at 35 µg
Developed using the ECL technique.
Predicted band size: 48 kDa
Ab13677 staining (0.05
µg/ml) of Human Heart lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Primary incubated for 1 hour.
ab13677 (3.8 µg/ml) staining ACADM/MCAD in paraffin-embedded human Liver. Steamed antigen retieval with citrate buffer (pH 6) was carried out.
ab13677 has been referenced in 2 publications.
- Wang F et al. PP56 improves energy homeostasis in a mouse model of pancreatic cancer. Tumour Biol 31:309-13 (2010). WB ; Mouse . PubMed: 20422342
- Powelka AM et al. Suppression of oxidative metabolism and mitochondrial biogenesis by the transcriptional corepressor RIP140 in mouse adipocytes. J Clin Invest 116:125-36 (2006). WB ; Mouse . PubMed: 16374519