Key features and details
- Rabbit polyclonal to ACADM/MCAD
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ACADM/MCAD antibody
See all ACADM/MCAD primary antibodies
DescriptionRabbit polyclonal to ACADM/MCAD
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
Recombinant fragment corresponding to Human ACADM/MCAD aa 1-93.
Database link: P11310
- 293T, A431, HeLa, HepG2 and Raji whole cell lysates; HeLa cells; Human DLD1 xenograft tissue.
Previously labelled as ACADM.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab152115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 47 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or perform heat mediated antigen retrieval with Tris-EDTA buffer pH 8.|
|ICC/IF||1/100 - 1/1000.|
FunctionThis enzyme is specific for acyl chain lengths of 4 to 16.
PathwayLipid metabolism; mitochondrial fatty acid beta-oxidation.
Involvement in diseaseDefects in ACADM are the cause of acyl-CoA dehydrogenase medium-chain deficiency (ACADMD) [MIM:201450]. It is an autosomal recessive disease which causes fasting hypoglycemia, hepatic dysfunction, and encephalopathy, often resulting in death in infancy.
Sequence similaritiesBelongs to the acyl-CoA dehydrogenase family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- ACAD 1 antibody
- ACAD1 antibody
- Acadm antibody
Anti-ACADM/MCAD antibody (ab152115) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 47 kDa
10% SDS PAGE
Immunofluorescent analysis of methanol-fixed HeLa cells labeling ACADM/MCAD with ab152115 at 1/100 dilution. Lower image shows cells co-stained with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded Human DLD1 xenograft tissue labeling ACADM/MCAD with ab152115 at 1/100 dilution.
ab152115 has not yet been referenced specifically in any publications.