Key features and details
- Rabbit polyclonal to ACADM/MCAD - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ACADM/MCAD antibody - C-terminal
See all ACADM/MCAD primary antibodies
DescriptionRabbit polyclonal to ACADM/MCAD - C-terminal
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Synthetic peptide within Human ACADM/MCAD (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: P11310
- WB: HeLa S3 whole cell lysate. IHC-P: Human ovarian cancer tissue. ICC/IF: SK-N-SH cells.
Previously labelled as ACADM.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab228769 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 47 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionThis enzyme is specific for acyl chain lengths of 4 to 16.
PathwayLipid metabolism; mitochondrial fatty acid beta-oxidation.
Involvement in diseaseDefects in ACADM are the cause of acyl-CoA dehydrogenase medium-chain deficiency (ACADMD) [MIM:201450]. It is an autosomal recessive disease which causes fasting hypoglycemia, hepatic dysfunction, and encephalopathy, often resulting in death in infancy.
Sequence similaritiesBelongs to the acyl-CoA dehydrogenase family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- ACAD 1 antibody
- ACAD1 antibody
- Acadm antibody
Anti-ACADM/MCAD antibody - C-terminal (ab228769) at 1/1000 dilution + HeLa S3 whole cell lysate at 30 µg
Predicted band size: 47 kDa
10% SDS-PAGE gel.
2% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells stained for ACADM/MCAD (green) using ab228769 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
Paraffin-embedded human ovarian cancer stained for ACADM/MCAD using ab228769 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228769 has not yet been referenced specifically in any publications.