Product nameAnti-ACADM/MCAD antibody [EPR3708]
See all ACADM/MCAD primary antibodies
DescriptionRabbit monoclonal [EPR3708] to ACADM/MCAD
Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human ACADM/MCAD aa 200-300. The exact sequence is proprietary.
- WB: Human heart or fetal liver tissue lysates; HeLa, HepG2, or K562 cell lysates. IHC-P: Human liver tissue. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab92461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended.
IP: 1/10 - 1/100.
WB: 1/10000 - 1/50000. Predicted molecular weight: 47 kDa.
Is unsuitable for Flow Cyt.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionThis enzyme is specific for acyl chain lengths of 4 to 16.
PathwayLipid metabolism; mitochondrial fatty acid beta-oxidation.
Involvement in diseaseDefects in ACADM are the cause of acyl-CoA dehydrogenase medium-chain deficiency (ACADMD) [MIM:201450]. It is an autosomal recessive disease which causes fasting hypoglycemia, hepatic dysfunction, and encephalopathy, often resulting in death in infancy.
Sequence similaritiesBelongs to the acyl-CoA dehydrogenase family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- ACAD 1 antibody
- ACAD1 antibody
- Acadm antibody
All lanes : Anti-ACADM/MCAD antibody [EPR3708] (ab92461) at 1/10000 dilution
Lane 1 : Human heart lysate
Lane 2 : fetal liver lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP conjugated Goat anti-Rabbit Ig at 1/2000 dilution
Predicted band size: 47 kDa
ab92461, at 1/100 dilution, staining ACADM/MCAD in formalin-fixed, paraffin-embedded Human liver tissue by immunohistochemistry.
Immunofluorescent staining of ACADM/MCAD in HeLa cells using ab92461 at 1/100 dilution.
Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ACADM/MCAD with unpurified ab92461 at 1/50 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
This product has been referenced in:
- Zheng F & Cai Y Concurrent exercise improves insulin resistance and nonalcoholic fatty liver disease by upregulating PPAR-? and genes involved in the beta-oxidation of fatty acids in ApoE-KO mice fed a high-fat diet. Lipids Health Dis 18:6 (2019). Read more (PubMed: 30611282) »
- Peng J et al. The detrimental effects of glucocorticoids exposure during pregnancy on offspring's cardiac functions mediated by hypermethylation of bone morphogenetic protein-4. Cell Death Dis 9:834 (2018). Read more (PubMed: 30082698) »