Recombinant Anti-ACADS/SCAD antibody [EPR10861(B)] (ab154823)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10861(B)] to ACADS/SCAD
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ACADS/SCAD antibody [EPR10861(B)]
See all ACADS/SCAD primary antibodies -
Description
Rabbit monoclonal [EPR10861(B)] to ACADS/SCAD -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa; HepG2 and 293T cell lysates; Human fetal heart and liver tissue lysates; 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10861(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 44 kDa. |
Target
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Pathway
Lipid metabolism; mitochondrial fatty acid beta-oxidation. -
Involvement in disease
Defects in ACADS are the cause of acyl-CoA dehydrogenase short-chain deficiency (ACADSD) [MIM:201470]. It is an autosomal recessive disorder resulting in acute acidosis and muscle weakness in infants, and a form of lipid-storage myopathy in adults. -
Sequence similarities
Belongs to the acyl-CoA dehydrogenase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 35 Human
- Entrez Gene: 11409 Mouse
- Entrez Gene: 64304 Rat
- Omim: 606885 Human
- SwissProt: P16219 Human
- SwissProt: Q07417 Mouse
- SwissProt: P15651 Rat
- Unigene: 507076 Human
see all -
Alternative names
- ACAD3 antibody
- ACADS antibody
- ACADS_HUMAN antibody
see all
Images
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All lanes : Anti-ACADS/SCAD antibody [EPR10861(B)] (ab154823) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Human fetal heart tissue lysate
Lane 5 : Human fetal liver tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 44 kDa -
Intracellular flow cytometric analysis of permeabilized 293T cells labeling ACADS/SCAD using ab154823 (red) at 1/10 dilution, or rabbit IgG (negative) (green).
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab154823 has been referenced in 8 publications.
- Park B et al. Pyruvate kinase M1 regulates butyrate metabolism in cancerous colonocytes. Sci Rep 12:8771 (2022). PubMed: 35610475
- Zhang B et al. Effects of riboflavin deficiency on the lipid metabolism of duck breeders and duck embryos. Poult Sci 100:101342 (2021). PubMed: 34438327
- Broniarek I et al. The Influence of Statins on the Aerobic Metabolism of Endothelial Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32098258
- Escudero S et al. Dynamic Regulation of Long-Chain Fatty Acid Oxidation by a Noncanonical Interaction between the MCL-1 BH3 Helix and VLCAD. Mol Cell 69:729-743.e7 (2018). PubMed: 29499131
- Koziel A & Jarmuszkiewicz W Hypoxia and aerobic metabolism adaptations of human endothelial cells. Pflugers Arch 469:815-827 (2017). PubMed: 28176017
- Tang J et al. Severe riboflavin deficiency induces alterations in the hepatic proteome of starter Pekin ducks. Br J Nutr 118:641-650 (2017). PubMed: 29185933
- Broniarek I et al. The effect of chronic exposure to high palmitic acid concentrations on the aerobic metabolism of human endothelial EA.hy926 cells. Pflugers Arch 468:1541-54 (2016). WB ; Human . PubMed: 27417103
- Meierhofer D et al. Ataxin-2 (Atxn2)-Knock-Out Mice Show Branched Chain Amino Acids and Fatty Acids Pathway Alterations. Mol Cell Proteomics 15:1728-39 (2016). PubMed: 26850065