Overview

  • Product name

    Anti-ACADS/SCAD antibody [EPR10861(B)]
    See all ACADS/SCAD primary antibodies
  • Description

    Rabbit monoclonal [EPR10861(B)] to ACADS/SCAD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human ACADS/SCAD (internal sequence).
    Database link: P16219

  • Positive control

    • HeLa; HepG2 and 293T cell lysates; Human fetal heart and liver tissue lysates; 293T cells.
  • General notes

     

     

    Previously labelled as ACADS. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab154823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Pathway

      Lipid metabolism; mitochondrial fatty acid beta-oxidation.
    • Involvement in disease

      Defects in ACADS are the cause of acyl-CoA dehydrogenase short-chain deficiency (ACADSD) [MIM:201470]. It is an autosomal recessive disorder resulting in acute acidosis and muscle weakness in infants, and a form of lipid-storage myopathy in adults.
    • Sequence similarities

      Belongs to the acyl-CoA dehydrogenase family.
    • Cellular localization

      Mitochondrion matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • ACAD3 antibody
      • ACADS antibody
      • ACADS_HUMAN antibody
      • Acyl Coenzyme A dehydrogenase, C2 to C3 short chain antibody
      • Acyl-CoA dehydrogenase, C2 to C3 short chain antibody
      • Acyl-CoA dehydrogenase, short chain antibody
      • Acyl-Coenzyme A dehydrogenase, short chain antibody
      • AI196007 antibody
      • Bcd-1 antibody
      • Bcd1 antibody
      • Butyryl CoA dehydrogenase antibody
      • Butyryl-CoA dehydrogenase antibody
      • EC 1.3.99.2 antibody
      • mitochondrial antibody
      • SCAD antibody
      • Short chain acyl CoA dehydrogenase antibody
      • Short-chain specific acyl-CoA dehydrogenase antibody
      • Short-chain specific acyl-CoA dehydrogenase, mitochondrial antibody
      • Unsaturated acyl CoA reductase antibody
      see all

    Images

    • All lanes : Anti-ACADS/SCAD antibody [EPR10861(B)] (ab154823) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Human fetal heart tissue lysate
      Lane 5 : Human fetal liver tissue lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 44 kDa

    • Flow cytometric analysis of permeabilized 293T cells labeling ACADS/SCAD using ab154823 (red) at 1/10 dilution, or rabbit IgG (negative) (green).

    References

    This product has been referenced in:

    • Koziel A & Jarmuszkiewicz W Hypoxia and aerobic metabolism adaptations of human endothelial cells. Pflugers Arch 469:815-827 (2017). Read more (PubMed: 28176017) »
    • Meierhofer D  et al. Ataxin-2 (Atxn2)-Knock-Out Mice Show Branched Chain Amino Acids and Fatty Acids Pathway Alterations. Mol Cell Proteomics 15:1728-39 (2016). Read more (PubMed: 26850065) »
    See all 3 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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