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Thanks for your help on the phone tonight, with my questions about the Anti-VLCAD antibody from Abcam (Mito Sciences), AB110285. We would like to find out where we expect to find distribution within mitochondria by thin-section TEM (LR-White Resin) in mitochondria that are apposed to other organelles in cells. Does one expect to see linear arrays of immunogold or Fluoronanogold (secondary antibody) probes along the inner membrane, or close to the membranes of the other organelles in contact with the mitochondria? Our experiment did not work with the VLCAD for either Santa Cruz' sample or your sample in heart. We use Fluoronanogold, so it did not show up by confocal or TEM. I appreciate your help.
Asked on Nov 21 2012
Thank you for contacting us. I wish to apologize for the wait.
We really do not know what you would expect to see with this product in EM, e.g. linear arrays probes along the inner membrane. Regarding whether this product should work with gold conjugation, we have not yet tried this but in our experience some Abs work really well, while others do not regardless of conjugation conditions.
I am very sorry that I could not be more helpful in this case. If you are interested in the AbTrial program please let me know. Please do not hesitate to contact us if you need any more advice or information.
Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447
Answered on Nov 21 2012