Overview

  • Product name

    Anti-ACADVL/VLCAD antibody
    See all ACADVL/VLCAD primary antibodies
  • Description

    Rabbit polyclonal to ACADVL/VLCAD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment corresponding to Human ACADVL/VLCAD aa 18-341.
    Database link: P49748

  • Positive control

    • H1299, HeLa, HepG2 and mouse liver whole cell lysates, HeLa cells, H358 xenograft
  • General notes

    Previously labelled as ACADVL. 

Properties

Applications

Our Abpromise guarantee covers the use of ab155138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 70 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Active toward esters of long-chain and very long chain fatty acids such as palmitoyl-CoA, mysritoyl-CoA and stearoyl-CoA. Can accomodate substrate acyl chain lengths as long as 24 carbons, but shows little activity for substrates of less than 12 carbons.
  • Pathway

    Lipid metabolism; mitochondrial fatty acid beta-oxidation.
  • Involvement in disease

    Defects in ACADVL are the cause of acyl-CoA dehydrogenase very long chain deficiency (ACADVLD) [MIM:201475]. ACADVLD is an autosomal recessive disease which leads to impaired long-chain fatty acid beta-oxidation. It is clinically heterogeneous, with three major phenotypes: a severe childhood form, with early onset, high mortality, and high incidence of cardiomyopathy; a milder childhood form, with later onset, usually with hypoketotic hypoglycemia as the main presenting feature, low mortality, and rare cardiomyopathy; and an adult form, with isolated skeletal muscle involvement, rhabdomyolysis, and myoglobinuria, usually triggered by exercise or fasting.
  • Sequence similarities

    Belongs to the acyl-CoA dehydrogenase family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACAD 6 antibody
    • ACAD6 antibody
    • ACADV_HUMAN antibody
    • Acadvl antibody
    • Acyl CoA dehydrogenase very long chain antibody
    • Acyl Coenzyme A dehydrogenase very long chain antibody
    • LCACD antibody
    • mitochondrial antibody
    • Very long chain specific acyl CoA dehydrogenase antibody
    • Very long chain specific acyl CoA dehydrogenase mitochondrial antibody
    • Very long-chain specific acyl-CoA dehydrogenase antibody
    • VLCAD antibody
    see all

Images

  • Anti-ACADVL/VLCAD antibody (ab155138) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 70 kDa



    7.5% SDS PAGE
  • Anti-ACADVL/VLCAD antibody (ab155138) at 1/3000 dilution + Mouse liver whole cell lysate at 50 µg

    Predicted band size: 70 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling ACADVL/VLCAD with ab155138 at 1/200. Image at the bottom costained with Hoechst 33342.

  • Immunohistochemical analysis of paraffin embedded H358 xenograft labeling ACADVL/VLCAD with ab155138 antibody at 1/250.

References

This product has been referenced in:

  • Kakimoto PA  et al. Resilient hepatic mitochondrial function and lack of iNOS dependence in diet-induced insulin resistance. PLoS One 14:e0211733 (2019). Read more (PubMed: 30716103) »
  • Chausse B  et al. Distinct metabolic patterns during microglial remodeling by oleate and palmitate. Biosci Rep 39:N/A (2019). Read more (PubMed: 30867255) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (human hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
human hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Dec 28 2017

Application
Western blot
Sample
Rat Cell lysate - other (Whole cell lysate of rat ST14a cells, and mitochon)
Gel Running Conditions
Reduced Denaturing (4-12% SDS-PAGE, Laemmli buffer with 10% β-Mercaptoethanol,10 min at 55C denaturation.)
Loading amount
20 µg
Specification
Whole cell lysate of rat ST14a cells, and mitochon
Blocking step
SEA Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C

Svitlana Yablonska

Verified customer

Submitted Aug 07 2017

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