Key features and details
- Rabbit polyclonal to ACAP1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ACAP1 antibody
DescriptionRabbit polyclonal to ACAP1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
Recombinant full length protein corresponding to Human ACAP1 aa 1-266.
MTVKLDFEECLKDSPRFRASIELVEAEVSELETRLEKLLKLGTGLLESGR HYLAASRAFVVGICDLARLGPPEPMMAECLEKFTVSLNHKLDSHAELLDA TQHTLQQQIQTLVKEGLRGFREARRDFWRGAESLEAALTHNAEVPRRRAQ EAEEAGAALRTARAGYRGRALDYALQINVIEDKRKFDIMEFVLRLVEAQA THFQQGHEELSRLSQYRKELGAQLHQLVLNSAREKRDMEQRHVLLKQKEL GGEEPEPSLREGPGGL
Database link: Q15027
- WB: Mouse lung lysate; Jurkat and K562 whole cell lysates. IHC-P: Human lung and pancreatic tissue.
This product was previously labelled as CENTB1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab235827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 82 kDa.|
FunctionGTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface.
Tissue specificityHighest level in lung and spleen. Low level in heart, kidney, liver and pancreas.
Sequence similaritiesContains 3 ANK repeats.
Contains 1 Arf-GAP domain.
Contains 1 BAR domain.
Contains 1 PH domain.
DomainPH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate ACAP1-binding to PIP2 or PIP3 containing membranes.
modificationsPhosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
- Information by UniProt
- ACAP 1 antibody
- ACAP1 antibody
- ACAP1_HUMAN antibody
All lanes : Anti-ACAP1 antibody (ab235827) at 1/1000 dilution
Lane 1 : Mouse lung tissue lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 82 kDa
Paraffin-embedded human lung tissue stained for ACAP1 using ab235827 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human pancreas tissue stained for ACAP1 using ab235827 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235827 has not yet been referenced specifically in any publications.