Product nameAnti-ACAP2 antibody - C-terminal
See all ACAP2 primary antibodies
DescriptionRabbit polyclonal to ACAP2 - C-terminal
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
- HeLa, 293T, Jurkat, TCMK-1, and NIH3T3 whole cell lysates.
This product was previously labelled as Centaurin beta 2
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab200583 was affinity purified using an epitope specific to ACAP2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab200583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 88 kDa.|
FunctionGTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6).
Tissue specificityWidely expressed. Highest level in lung.
Sequence similaritiesContains 3 ANK repeats.
Contains 1 Arf-GAP domain.
Contains 1 BAR domain.
Contains 1 PH domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- ACAP 2 antibody
- ACAP2 antibody
- ACAP2_HUMAN antibody
All lanes : Anti-ACAP2 antibody - C-terminal (ab200583) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 88 kDa
Exposure time: 3 minutes
Detection of ACAP2 in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab200583 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection (Lane 1). Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab200583 has not yet been referenced specifically in any publications.