Overview

  • Product name

    Anti-ACAP2 antibody [EPR15721]
    See all ACAP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR15721] to ACAP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ACAP2 aa 650-750. The exact sequence is proprietary.
    Database link: Q15057

  • Positive control

    • HeLa, Jurkat, 293, MOLT4, Human fetal lung, Human fetal kidney, RAW 264.7 and PC12 lysates; Jurkat cells.
  • General notes

     

     

     This product was previously labelled as Centaurin beta 2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15721
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
Flow Cyt 1/270.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6).
  • Tissue specificity

    Widely expressed. Highest level in lung.
  • Sequence similarities

    Contains 3 ANK repeats.
    Contains 1 Arf-GAP domain.
    Contains 1 BAR domain.
    Contains 1 PH domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACAP 2 antibody
    • ACAP2 antibody
    • ACAP2_HUMAN antibody
    • ANK repeat and PH domain-containing protein 2 antibody
    • Arf GAP with coiled coil ANK repeat and PH domain containing protein 2 antibody
    • Arf GAP with coiled coil ANK repeat and PH domains 2 antibody
    • Arf-GAP with coiled-coil antibody
    • ArfGAP with coiled coil ankyrin repeat and PH domains 2 antibody
    • CENT B 2 antibody
    • Centaurin beta2 antibody
    • Centaurin-beta-2 antibody
    • CENTB 2 antibody
    • CENTB2 antibody
    • Cnt b2 antibody
    • Cnt-b2 antibody
    • Cntb2 antibody
    • KIAA0041 antibody
    • Similar to yeast ZINC FINGER PROTEIN GCS1 antibody
    see all

Images

  • All lanes : Anti-ACAP2 antibody [EPR15721] (ab191424) at 1/5000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : Jurkat lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 88 kDa

  • Flow Cytometric analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells (paraformaldehyde-fixed, 2%) labeling ACAP2 with ab191424 at 1/270 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • All lanes : Anti-ACAP2 antibody [EPR15721] (ab191424) at 1/5000 dilution

    Lane 1 : 293 lysate
    Lane 2 : MOLT4 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 88 kDa

  • All lanes : Anti-ACAP2 antibody [EPR15721] (ab191424) at 1/5000 dilution

    Lane 1 : Human fetal lung lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 88 kDa

  • All lanes : Anti-ACAP2 antibody [EPR15721] (ab191424) at 1/1000 dilution

    Lane 1 : RAW 264.7 lysate
    Lane 2 : PC12 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 88 kDa

References

ab191424 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191424.
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