Recombinant Anti-ACAP2 antibody [EPR15721] - BSA and Azide free (ab232615)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ACAP2 antibody [EPR15721] - BSA and Azide free
See all ACAP2 primary antibodies -
Description
Rabbit monoclonal [EPR15721] to ACAP2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human ACAP2 aa 650-750. The exact sequence is proprietary.
Database link: Q15057 -
Positive control
- Flow Cyt: Jurkat cells.
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General notes
ab232615 is the carrier-free version of ab191424 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab232615 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Centaurin beta 2
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15721 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab232615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | Use at an assay dependent concentration. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa). |
Target
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Function
GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6). -
Tissue specificity
Widely expressed. Highest level in lung. -
Sequence similarities
Contains 3 ANK repeats.
Contains 1 Arf-GAP domain.
Contains 1 BAR domain.
Contains 1 PH domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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Database links
- Entrez Gene: 23527 Human
- Entrez Gene: 78618 Mouse
- Entrez Gene: 619382 Rat
- Omim: 607766 Human
- SwissProt: Q15057 Human
- SwissProt: Q6ZQK5 Mouse
- SwissProt: Q5FVC7 Rat
- Unigene: 593373 Human
see all -
Alternative names
- ACAP 2 antibody
- ACAP2 antibody
- ACAP2_HUMAN antibody
see all
Images
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![Flow Cytometry - Anti-ACAP2 antibody [EPR15721] - BSA and Azide free (ab232615)](https://www.abcam.com/ps/products/232/ab232615/Images/ab232615-308940-2326151.jpg)
Flow Cytometry - Anti-ACAP2 antibody [EPR15721] - BSA and Azide free (ab232615)Flow Cytometric analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells (paraformaldehyde-fixed, 2%) labeling ACAP2 with ab191424 at 1/270 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191424).
Datasheets and documents
References
ab232615 has not yet been referenced specifically in any publications.
