Overview

  • Product name
    Anti-ACAT1 antibody [EPR10359]
    See all ACAT1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10359] to ACAT1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human ACAT1 (P24752).

  • Positive control
    • Human fetal liver, HepG2, (C) SKBR-3, THP-1 and Human fetal kidney lysates, Human heart & liver tissue, immunoprecipitation pellet from Human fetal liver lysate
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab168342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 45 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Plays a major role in ketone body metabolism.
    • Involvement in disease
      Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD) [MIM:203750]; also known as alpha-methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype.
    • Sequence similarities
      Belongs to the thiolase family.
    • Cellular localization
      Mitochondrion.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACAT 1 antibody
      • ACAT antibody
      • acat1 antibody
      • Acetoacetyl CoA thiolase antibody
      • acetoacetyl Coenzyme A thiolase antibody
      • Acetoacetyl-CoA thiolase antibody
      • Acetyl CoA acetyltransferase, mitochondrial antibody
      • Acetyl Coenzyme A acetyltransferase 1 antibody
      • Acetyl-CoA acetyltransferase antibody
      • acetyl-coa acetyltransferase precursor, mitochondrial antibody
      • Acetyl-CoA thiolase, mitochondrial antibody
      • acetyl-Coenzyme A acetyltransferase 1 antibody
      • MAT antibody
      • mitochondrial acetoacetyl-CoA thiolase antibody
      • mitochondrial antibody
      • RATACAL antibody
      • T2 antibody
      • testicular tissue protein Li 198 antibody
      • THIL antibody
      • THIL_HUMAN antibody
      see all

    Images

    • All lanes : Anti-ACAT1 antibody [EPR10359] (ab168342) at 1/1000 dilution

      Lane 1 : Human fetal liver lysates
      Lane 2 : HepG2 lysates
      Lane 3 : SKBR-3 lysates
      Lane 4 : THP-1 lysates
      Lane 5 : Human fetal kidney lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 45 kDa

    • ab168342 showing +ve staining in Human ovarian carcinoma tissue.

    • ab168342 showing +ve staining in Human normal kidney tissue.

    • ab168342 showing +ve staining in Human normal colon tissue.

    • ab168342 showing +ve staining in Human papillary adenocarcinoma of thyroid gland tissue.

    • Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling ACAT1 with ab168342 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ACAT1 with ab168342 at 1/50 dilution.
    • Western blot analysis labelling ACAT1 on immunoprecipitation pellet from Human fetal liver lysate using ab168342 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    This product has been referenced in:
    • Chen X  et al. Comparative profiling of analog targets: a case study on resveratrol for mouse melanoma metastasis suppression. Theranostics 8:3504-3516 (2018). Read more (PubMed: 30026862) »
    • Mitra R  et al. Positive regulation of prostate cancer cell growth by lipid droplet forming and processing enzymes DGAT1 and ABHD5. BMC Cancer 17:631 (2017). Read more (PubMed: 28877685) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample
    Mouse Cell (Retina: GCL,OPL, POS)
    Specification
    Retina: GCL,OPL, POS
    Permeabilization
    No
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Feb 24 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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