Recombinant
RabMAb

Recombinant Anti-ACAT1 antibody [EPR10359] - BSA and Azide free (ab240152)

Overview

  • Product name

    Anti-ACAT1 antibody [EPR10359] - BSA and Azide free
    See all ACAT1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10359] to ACAT1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ACAT1. The exact sequence is proprietary.

  • General notes

    Ab240152 is the carrier-free version of ab168342. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240152 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Plays a major role in ketone body metabolism.
    • Involvement in disease

      Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD) [MIM:203750]; also known as alpha-methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype.
    • Sequence similarities

      Belongs to the thiolase family.
    • Cellular localization

      Mitochondrion.
    • Information by UniProt
    • Database links

    • Alternative names

      • ACAT 1 antibody
      • ACAT antibody
      • acat1 antibody
      • Acetoacetyl CoA thiolase antibody
      • acetoacetyl Coenzyme A thiolase antibody
      • Acetoacetyl-CoA thiolase antibody
      • Acetyl CoA acetyltransferase, mitochondrial antibody
      • Acetyl Coenzyme A acetyltransferase 1 antibody
      • Acetyl-CoA acetyltransferase antibody
      • acetyl-coa acetyltransferase precursor, mitochondrial antibody
      • Acetyl-CoA thiolase, mitochondrial antibody
      • acetyl-Coenzyme A acetyltransferase 1 antibody
      • MAT antibody
      • mitochondrial acetoacetyl-CoA thiolase antibody
      • mitochondrial antibody
      • RATACAL antibody
      • T2 antibody
      • testicular tissue protein Li 198 antibody
      • THIL antibody
      • THIL_HUMAN antibody
      see all

    Images

    • ab168342 showing +ve staining in Human ovarian carcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168342).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab168342 showing +ve staining in Human normal kidney tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168342).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab168342 showing +ve staining in Human normal colon tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168342).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab168342 showing +ve staining in Human papillary adenocarcinoma of thyroid gland tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168342).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling ACAT1 with ab168342 at 1/50 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168342).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ACAT1 with ab168342 at 1/50 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168342).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot analysis labelling ACAT1 on immunoprecipitation pellet from Human fetal liver lysate using ab168342 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168342).

    References

    ab240152 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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