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Rabbit polyclonal to ACAT2
Predicted to work with:
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human ACAT2.
(Peptide available as
This antibody gave a positive signal in human liver tissue lysate as well as the following whole cell lysates: HepG2; HEK293; K562.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
Belongs to the thiolase family.
Information by UniProt
acetoacetyl CoA thiolase antibody
acetoacetyl Coenzyme A thiolase antibody
Western blot - Anti-ACAT2 antibody (ab74901)
All lanes :
Anti-ACAT2 antibody (ab74901) at 1 µg/ml
Lane 1 :
Human liver tissue lysate - total protein (
Lane 2 :
HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 :
K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 4 :
HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
Immunocytochemistry/ Immunofluorescence - Anti-ACAT2 antibody (ab74901)
ICC/IF image of ab74901 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74901, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 4% PFA fixed (10 min) Hek293 cells at 5µg/ml.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"