Anti-ACAT2/Acetyl-CoA acetyltransferase antibody (ab187712)

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Western blot - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody (ab187712)

    Key features and details

    • Rabbit polyclonal to ACAT2/Acetyl-CoA acetyltransferase
    • Suitable for: WB
    • Reacts with: Mouse, Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-ACAT2/Acetyl-CoA acetyltransferase antibody
      See all ACAT2/Acetyl-CoA acetyltransferase primary antibodies

    • Description

      Rabbit polyclonal to ACAT2/Acetyl-CoA acetyltransferase

    • Host species

      Rabbit

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Mouse, Human
      Predicted to work with: Rhesus monkey, Gorilla, Orangutan

    • Immunogen

      Synthetic peptide within Human ACAT2/Acetyl-CoA acetyltransferase aa 150-200 (internal sequence). The exact sequence is proprietary.
      Sequence:

      GLTDAFHNCHMGITAENVAKKWQVSREDQDKVAVLSQNRTENAQKAGHFD K


      Database link: Q9BWD1
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • HeLa, 293T, Jurkat, mouse TCMK-1 and mouse NIH 3T3 whole cell lysates.

    • General notes

       This product was previously labelled as ACAT2, Acetyl-CoA acetyltransferase

       

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab187712 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/2000 - 1/10000. Predicted molecular weight: 41 kDa.

    Target

    Images

    • Western blot - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody (ab187712)
      Western blot - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody (ab187712)
      All lanes : Anti-ACAT2/Acetyl-CoA acetyltransferase antibody (ab187712) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate
      Lane 2 : 293T whole cell lysate
      Lane 3 : Jurkat whole cell lysate
      Lane 4 : Mouse TCMK-1 whole cell lysate
      Lane 5 : Mouse NIH 3T3 whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 41 kDa


      Exposure time: 3 minutes

    References (0)

    Publishing research using ab187712? Please let us know so that we can cite the reference in this datasheet.

    ab187712 has not yet been referenced specifically in any publications.

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    Western blot abreview for Anti-ACAT2/Acetyl-CoA acetyltransferase antibody

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Duodenum)
    Gel Running Conditions
    Reduced Denaturing (12.5%)
    Loading amount
    30 µg
    Specification
    Duodenum
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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    Verified customer

    Submitted May 04 2017

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