Application
Western blot
Sample
Mouse Tissue lysate - whole (Duodenum)
Gel Running Conditions
Reduced Denaturing (12.5%)
Loading amount
30 µg
Specification
Duodenum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% NFDM in TBST
Dilution
1/2000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: goat anti rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
3 second(s)
Bands
Specific: 41 kDa Non-specific: 25, 50, 70, 150 kDa
Positive control
Duodenum Tissue Lysate
Additional data
Additional Notes
Lanes
1: Marker
2: C57BLJ6 Duodenum Whole Tissue Lysate
3: C57BLJ6 Duodenum Cytosolic Fraction
4: C57BLJ6 Duodenum Microsomal Fraction
5: C57BLJ6 Duodenum Brush Border Fraction
6: Empty
7: Transgenic Mouse Duodenum Whole Tissue Lysate
8: Transgenic Mouse Duodenum Cytosolic Fraction
9: Transgenic Mouse Duodenum Microsomal Fraction
10: Transgenic Mouse Duodenum Brush Border Fraction
Molecular Weight marker (kDa)
250
150
100
75
50
37
25
20
15
10
Abcam user community
Verified customer
Submitted May 04 2017