Recombinant
RabMAb

Recombinant Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] (ab191431)

Overview

  • Product name

    Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)]
    See all ACAT2/Acetyl-CoA acetyltransferase primary antibodies
  • Description

    Rabbit monoclonal [EPR8416(2)] to ACAT2/Acetyl-CoA acetyltransferase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ACAT2/Acetyl-CoA acetyltransferase aa 150-250. The exact sequence is proprietary.
    Database link: Q9BWD1

  • Positive control

    • HepG2, HeLa, Jurkat, K562, PC12 and NIH 3T3 cell lysates.
  • General notes

     

     

     This product was previously labelled as ACAT2, Acetyl-CoA acetyltransferase

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab191431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
IP 1/20.

Target

Images

  • All lanes : Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] (ab191431) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 41 kDa

  • Western blot analysis of immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab191431 at 1/20 dilution (lane 1) or PBS control (lane 2).
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • All lanes : Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] (ab191431) at 1/1000 dilution

    Lane 1 : PC12 cell lysate
    Lane 2 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 41 kDa

References

ab191431 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab191431.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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