Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8623] to ACBD3
- Suitable for: WB, IP, Flow Cyt
- Knockout validated
- Reacts with: Human
Product nameAnti-ACBD3 antibody [EPR8623]
See all ACBD3 primary antibodies
DescriptionRabbit monoclonal [EPR8623] to ACBD3
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Human
A synthetic peptide corresponding to a region within Human ACBD3 (UniProt ID: Q9H3P7).
- K562, HeLa, 293T and LnCaP cell lysates. K562 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 2.28 x 10 -10 M Learn more about KD
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 61 kDa.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionInvolved in the maintenance of Golgi structure by interacting with giantin, affecting protein transport between the endoplasmic reticulum and Golgi. Involved in hormone-induced steroid biosynthesis in testicular Leydig cells.
Tissue specificityUbiquitous, with highest expression in testis and ovary.
Sequence similaritiesContains 1 ACB (acyl-CoA-binding) domain.
Contains 1 GOLD domain.
DomainThe GOLD domain is essential for giantin binding.
Cellular localizationGolgi apparatus membrane. Mitochondrion. Also mitochondrial (via its interaction with PBR).
- Information by UniProt
- ACBD 3 antibody
- ACBD3 antibody
- Acyl CoA binding domain containing protein 3 antibody
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: ACBD3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab134952 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab134952 was shown to specifically react with ACBD3 when ACBD3 knockout samples were used. Wild-type and ACBD3 knockout samples were subjected to SDS-PAGE. Ab134952 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-ACBD3 antibody [EPR8623] (ab134952) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : LNCaP cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 61 kDa
Flow cytometric analysis of permeabilized K562 cells labelling ACBD3 using ab134952 (red) at 1/100 dilution and rabbit IgG negative control (green).
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab134952 has been referenced in 1 publication.
- Lu MC et al. CPUY192018, a potent inhibitor of the Keap1-Nrf2 protein-protein interaction, alleviates renal inflammation in mice by restricting oxidative stress and NF-?B activation. Redox Biol 26:101266 (2019). PubMed: 31279986