Overview

  • Product name

    Anti-ACBD3 antibody [EPR8623]
    See all ACBD3 primary antibodies
  • Description

    Rabbit monoclonal [EPR8623] to ACBD3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to a region within Human ACBD3 (UniProt ID: Q9H3P7).

  • Positive control

    • K562, HeLa, 293T and LnCaP cell lysates. K562 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      Involved in the maintenance of Golgi structure by interacting with giantin, affecting protein transport between the endoplasmic reticulum and Golgi. Involved in hormone-induced steroid biosynthesis in testicular Leydig cells.
    • Tissue specificity

      Ubiquitous, with highest expression in testis and ovary.
    • Sequence similarities

      Contains 1 ACB (acyl-CoA-binding) domain.
      Contains 1 GOLD domain.
    • Domain

      The GOLD domain is essential for giantin binding.
    • Cellular localization

      Golgi apparatus membrane. Mitochondrion. Also mitochondrial (via its interaction with PBR).
    • Information by UniProt
    • Database links

    • Alternative names

      • ACBD 3 antibody
      • ACBD3 antibody
      • Acyl CoA binding domain containing protein 3 antibody
      • Acyl Coenzyme A binding domain containing 3 antibody
      • Acyl-CoA-binding domain-containing protein 3 antibody
      • GCP 60 antibody
      • GCP60 antibody
      • GCP60_HUMAN antibody
      • GOCAP 1 antibody
      • GOCAP1 antibody
      • Golgi complex associated protein 1 60kDa antibody
      • Golgi complex associated protein 1 antibody
      • Golgi complex-associated protein 1 antibody
      • Golgi phosphoprotein 1 antibody
      • Golgi resident protein GCP60 antibody
      • GOLPH 1 antibody
      • GOLPH1 antibody
      • PAP 7 antibody
      • PAP7 antibody
      • PBR and PKA associated protein 7 antibody
      • PBR associated protein antibody
      • PBR- and PKA-associated protein 7 antibody
      • Peripheral benzodiazepine receptor associated protein PAP7 antibody
      • Peripheral benzodiazepine receptor-associated protein PAP7 antibody
      • Peripherial benzodiazepine receptor associated protein antibody
      • PKA (RIalpha) associated protein antibody
      see all

    Images

    • Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: ACBD3 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: HepG2 whole cell lysate (20 µg)

       Lanes 1 - 4: Merged signal (red and green). Green - ab134952 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

       ab134952 was shown to specifically react with ACBD3 when ACBD3 knockout samples were used. Wild-type and ACBD3 knockout samples were subjected to SDS-PAGE. Ab134952 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-ACBD3 antibody [EPR8623] (ab134952) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : LNCaP cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size: 61 kDa

    • Flow cytometric analysis of permeabilized K562 cells labelling ACBD3 using ab134952 (red) at 1/100 dilution and rabbit IgG negative control (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab134952 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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