Anti-ACD antibody (ab112050)
Key features and details
- Rabbit polyclonal to ACD
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ACD antibody
See all ACD primary antibodies -
Description
Rabbit polyclonal to ACD -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan -
Immunogen
Synthetic peptide corresponding to Human ACD aa 150-200. (GeneID 65057)
Database link: NP_001075955.1 -
Positive control
- HeLa and Jurkat whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab112050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 58 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 58 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Promotes binding of POT1 to single-stranded telomeric DNA. Modulates the inhibitory effects of POT1 on telomere elongation. The ACD-POT1 heterodimer enhances telomer elongation by increasing telomerase processivity. Plays a role in shelterin complex assembly. May play a role in organogenesis. -
Cellular localization
Nucleus. Chromosome > telomere. - Information by UniProt
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Database links
- Entrez Gene: 65057 Human
- Entrez Gene: 307798 Rat
- Omim: 609377 Human
- SwissProt: Q96AP0 Human
- SwissProt: Q4FZR5 Rat
- Unigene: 78019 Human
- Unigene: 8611 Rat
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Alternative names
- Acd antibody
- ACD_HUMAN antibody
- Adrenocortical dysplasia homolog antibody
see all
Images
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All lanes : Anti-ACD antibody (ab112050) at 0.4 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 5 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 58 kDa
Exposure time: 3 minutes -
ab112050 at 1 µg/ml staining PDE3A in HeLa cell lysate immunoprecipitated using ab112050 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: controle IgG. Predicted band size : 58 kDa Exposure time: 30 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab112050 has been referenced in 1 publication.
- Smith EW et al. Insights into POT1 structural dynamics revealed by cryo-EM. PLoS One 17:e0264073 (2022). PubMed: 35176105