Overview

  • Product name

    ACE Assay Kit (Angiotensin I Converting Enzyme)
    See all Angiotensin Converting Enzyme 1 kits
  • Detection method

    Fluorescent
  • Sample type

    Serum, Plasma, Cell culture extracts, Tissue
  • Assay duration

    One step assay
  • Product overview

    ACE Assay Kit (Angiotensin I Converting Enzyme) (ab239703) utilizes an active ACE1 to cleave a synthetic o-aminobenzoyl peptide (Abz-based peptide) substrate to release a fluorophore. The released Abz can be easily quantified using a fluorescence microplate reader. This assay kit is simple, rapid and can detect ACE activity as low as 10 mU in biological samples, such as lung, heart and kidney tissue, and serum and plasma. It can also be used to determine the  enzymatic activity of purified ACE1. 

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Abz-Standard (1 mM) 1 x 100µl
    ACE1 Assay Buffer 1 x 20ml
    ACE1 Dilution Buffer 1 x 1ml
    ACE1 Lysis Buffer 1 x 40ml
    ACE1 Positive control 1 x 5µl
    ACE1 Substrate 1 x 300µl
  • Research areas

  • Function

    Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
  • Tissue specificity

    Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis.
  • Involvement in disease

    Ischemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
    Renal tubular dysgenesis (RTD) [MIM:267430]: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Note=The disease is caused by mutations affecting the gene represented in this entry.
    Microvascular complications of diabetes 3 (MVCD3) [MIM:612624]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
    Intracerebral hemorrhage (ICH) [MIM:614519]: A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
  • Sequence similarities

    Belongs to the peptidase M2 family.
  • Post-translational
    modifications

    Phosphorylated by CK2 on Ser-1299; which allows membrane retention.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Alternative names

    • ACE
    • ACE 1
    • ACE T
    • ACE_HUMAN
    • ACE1
    • Angiotensin converting enzyme somatic isoform
    • Angiotensin converting enzyme testis specific isoform
    • Angiotensin I converting enzyme
    • Angiotensin I converting enzyme 1
    • Angiotensin I converting enzyme peptidyl dipeptidase A 1
    • angiotensin I converting enzyme peptidyl-dipeptidase A 1 transcript
    • Angiotensin-converting enzyme
    • Carboxycathepsin
    • CD 143
    • CD143
    • CD143 antigen
    • DCP
    • DCP 1
    • DCP1
    • Dipeptidyl carboxypeptidase 1
    • Dipeptidyl carboxypeptidase I
    • Kininase II
    • MGC26566
    • MVCD3
    • Peptidase P
    • Peptidyl dipeptidase A
    • soluble form
    • Testicular ECA
    see all

Images

  • Abz-Standard Curve (0-1000 pmol), error bars indicate SD (n=3).

  • Kinetic activity curves using different amounts of ACE1. Positive Control in the assay.

  • ACE1 activity was measured for different types of rat tissue samples (lung, heart and kidney; 0.6 µg each). Assays were performed following the kit protocol.

Protocols

References

ab239703 has not yet been referenced specifically in any publications.

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